defA
168
formylmethionine deformylase, binds and inhibits [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] during oxidative stress
locus
BSU_15720
Molecular weight
17.63 kDa
pI
4.73
function
translation
product
formylmethionine deformylase
essential
no
ec
3.5.1.88
synonyms
defA, yloK, def
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0242 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,646,512 1,646,994
Phenotypes of a mutant
a ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant is not viable unless the cells acquire suppressor mutations in ''[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt], [gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]'' or ''[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]'' [Pubmed|27983482]
The protein
Catalyzed reaction/ biological activity
H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)
binds and inhibits [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] during oxidative stress [pubmed|38857388]
Protein family
polypeptide deformylase family (with [protein|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB], according to UniProt)
Structure
[PDB|1WS0] (from B.cereus, 67% identity) [pubmed|16049914]
[AF|P94462]
Paralogous protein(s)
[protein|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]
Expression and Regulation
Operons
genes
[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[Pubmed|16964327]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
Biological materials
Mutant
BKE15720 ([gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG
BKK15720 ([gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG
References
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Time of last update: 2024-10-14 18:47:02
Author of last update: Jstuelk