defA

defA
168

formylmethionine deformylase, binds and inhibits [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] during oxidative stress

locus
BSU_15720
Molecular weight
17.63 kDa
pI
4.73
Protein length
160 aaSequenceBlast
Gene length
483 bpSequenceBlast
function
translation
product
formylmethionine deformylase
essential
no
ec
3.5.1.88
synonyms
defA, yloK, def
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0242 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,646,512  1,646,994
Phenotypes of a mutant
a ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant is not viable unless the cells acquire suppressor mutations in ''[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt], [gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]'' or ''[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]'' [Pubmed|27983482]
The protein
Catalyzed reaction/ biological activity
H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)
binds and inhibits [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] during oxidative stress [pubmed|38857388]
Protein family
polypeptide deformylase family (with [protein|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB], according to UniProt)
Structure
[PDB|1WS0] (from B.cereus, 67% identity) [pubmed|16049914]
[AF|P94462]
Paralogous protein(s)
[protein|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]
Expression and Regulation
Operons
genes
[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[Pubmed|16964327]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]→[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-12-21 11:48:06

Jstuelk

75

d80326080a97255f868ab0fdec860faa54d5674c

244CB061EA23DF7106827650C1723DD251F3D15F

genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
Open in new tab

[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]->[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-12-21 12:16:10

Jstuelk

138

67080d1e476b4c1544f46abe5db9b45c3ccd93f3

2C39B75EDCD294B0CAC1DECB4007285A35FDAE1D

Biological materials
Mutant
BKE15720 ([gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA,  downstream forward: _UP4_CTAGCGGATATGGAAGGATG
BKK15720 ([gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA,  downstream forward: _UP4_CTAGCGGATATGGAAGGATG
References
11429456,12627383,18288106,16964327,23770820,27983482,16049914,38857388

3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA

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Time of last update: 2024-12-19 23:02:22

Author of last update: Jstuelk