mntA
168
manganese [wiki|ABC transporter] (binding protein, lipoprotein)
locus
BSU_30770
Molecular weight
33.26 kDa
pI
6.16
function
manganese uptake
product
manganese [wiki|ABC transporter] (binding protein)
essential
no
synonyms
mntA, ytgA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0803 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,145,038 3,145,958
Phenotypes of a mutant
essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que and Helmann, 2000]
knockdown of expression results in loss of [category|SW.5.2.1|ICEBs1] conjugation (both in donor cells) [pubmed|35490406]
The protein
Protein family
bacterial solute-binding protein 9 family (with [protein|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA], according to UniProt)
Structure
[PDB|1TOA] (zinc-binding protein from Treponema pallidum, 46% identity) [pubmed|10404217]
[AF|O34385]
Paralogous protein(s)
[protein|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA]
[wiki|Localization]
cell membrane (according to Swiss-Prot), extracellular lipoprotein (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]-[gene|7821C82C6C30079F713B84F1E4D654E07CE5072E|mntB]-[gene|1B7C4AD6CD0632051A0E8CA57034872D13FF67F9|mntC]-[gene|F7DA077FE47DBB409BE2B1D9F12A9D719C246166|mntD]
description
[Pubmed|10760146]
regulation
repressed at high Mn(II) concentrations ([protein|search|MntR]) [Pubmed|12950915]
regulatory mechanism
[protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]: repression, [Pubmed|12950915], in [regulon|protein:FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|mntA]' and '[protein|search|menC]' [PubMed|20525796]
Biological materials
Mutant
MGNA-A285 (ytgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/285 NBRP B. subtilis, Japan]
BKE30770 ([gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG, downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA
BKK30770 ([gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG, downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-30 02:51:43
Author of last update: Jstuelk