murC
168
UDP-N-acetylmuramoyl-L-alanine synthetase
locus
BSU_29790
Molecular weight
48.20 kDa
pI
5.31
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylmuramoyl-L-alanine synthetase
essential
yes
ec
6.3.2.8
synonyms
murC, ytxF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0773 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,048,177 3,049,475
Phenotypes of a mutant
essential [Pubmed|12682299]
murC is dispensable in wall-less L-forms [Pubmed|24704074]
The protein
Catalyzed reaction/ biological activity
ATP + L-alanine + UDP-N-acetyl-α-D-muramate --> ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanine (according to UniProt)
Protein family
[wiki|MurCDEF family] (according to UniProt)
Structure
[PDB|1GQQ] (from Haemophilus influenzae, 31% identity)
[AF|P40778]
[wiki|Localization]
cytoplasm (Homogeneous) [Pubmed|16479537]
forms banded patterns along the length of the cell, this pattern formation depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [Pubmed|20233306]
Expression and Regulation
Operons
genes
[gene|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]-[gene|3BF530AB1B6A3DB3671FC046E2B38CA1936E6F78|murC]
description
[Pubmed|22383849]
Biological materials
Expression vectors
pGP3746 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP4406 [gene|3BF530AB1B6A3DB3671FC046E2B38CA1936E6F78|murC]-3xFLAG spc (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3743 (in [wiki|pAC5]), available in [wiki|Jrög Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-29 05:43:52
Author of last update: Jstuelk