yxaL
168
similar to Ser/Thr kinase, increases the processivity of the [protein|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] helicase
locus
BSU_39940
Molecular weight
43.72 kDa
pI
6.38
function
unknown
product
unknown
essential
no
synonyms
yxaL, yxaK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1520 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,102,429 4,103,661
The protein
Structure
[PDB|3HXJ] (from Methanococcus maripaludis, 33% Identity)
[AF|P42111]
[wiki|Localization]
extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]
description
[pubmed|38294221]
genes
[gene|0649520164CCC6120C40CE15EF08994BE0E79A25|yxaJ]-[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]
description
[Pubmed|10746760]
regulation
repressed by [protein|search|Rok] [Pubmed|15743949]
regulatory mechanism
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|27902860,15743949], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675,18840696], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
Biological materials
Mutant
MGNA-B687 (yxaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1686 NBRP B. subtilis, Japan]
BKE39940 ([gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTGAGACCCCCATTA, downstream forward: _UP4_TAACTGAATAGAATAGCAGA
BKK39940 ([gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTGAGACCCCCATTA, downstream forward: _UP4_TAACTGAATAGAATAGCAGA
Expression vectors
pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP824, available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
References
Page visits: 3898
Time of last update: 2024-12-21 00:29:48
Author of last update: Jstuelk