yxaL

yxaL
168

similar to Ser/Thr kinase, increases the processivity of the [protein|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] helicase

locus
BSU_39940
Molecular weight
43.72 kDa
pI
6.38
Protein length
Gene length
function
unknown
product
unknown
essential
no
synonyms
yxaL, yxaK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1520 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,102,429  4,103,661
The protein
Structure
[PDB|3HXJ] (from Methanococcus maripaludis, 33% Identity)
[AF|P42111]
[wiki|Localization]
extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]
description
[pubmed|38294221]
Open in new tab

[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]

2024-10-09 02:23:22

Jstuelk

32

93D6EB6FDD5C62F6C8F1D96DB255716AAA0E0617

93D6EB6FDD5C62F6C8F1D96DB255716AAA0E0617

genes
[gene|0649520164CCC6120C40CE15EF08994BE0E79A25|yxaJ]-[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]
description
[Pubmed|10746760]
regulation
repressed by [protein|search|Rok] [Pubmed|15743949]
regulatory mechanism
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|27902860,15743949], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675,18840696], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
Open in new tab

[gene|0649520164CCC6120C40CE15EF08994BE0E79A25|yxaJ]→[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]

2024-09-27 09:21:20

ghost

74

46dd68da7d5f32ee653b610adfe637b57da7f7c1

ED645E313FCA048AA98BE18895E79D2AFE355ECD

Biological materials
Mutant
MGNA-B687 (yxaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1686 NBRP B. subtilis, Japan]
BKE39940 ([gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATTGAGACCCCCATTA,  downstream forward: _UP4_TAACTGAATAGAATAGCAGA
BKK39940 ([gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39940 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATTGAGACCCCCATTA,  downstream forward: _UP4_TAACTGAATAGAATAGCAGA
Expression vectors
pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP824, available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
References
12073041,10746760,15743949,18957862,31022189,34279225,38294221

3BDC62A14BEEAEFD8E075E0958CAE41377808922

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Time of last update: 2024-10-09 07:42:12

Author of last update: Jstuelk