aexA
168
"sleeping beauty" amino acid exporter
locus
BSU_05160
Molecular weight
35.16 kDa
pI
9.62
function
export of toxic/ growth-inhibiting amino acids
product
amino acid exporter A
essential
no
ec
null
synonyms
ydeD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
562,502 563,461
The protein
Catalyzed reaction/ biological activity
export of asparagine [pubmed|38193659]
export of L-alanine [pubmed|38470260]
export of ß-alanine [pubmed|38470260]
export of serine [pubmed|38470260]
export of glycine [pubmed|38470260]
Protein family
[wiki|EamA transporter family] (according to UniProt)
[wiki|Domains]
2[wiki|EamA domain]s (aa 18-149, aa 171-296) (according to UniProt)
Structure
[AF|P96661]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|3BD05DE3ED5B293980F47EB621C54C7339F78DC7|aexA]
regulation
([protein|24A86A4846187E6026A2B7645DC4B7C70908942E|aerA]): transcription activation [pubmed|38470260]
expression
weak induction in the presence of 2,3-diaminopropionic acid ([protein|24A86A4846187E6026A2B7645DC4B7C70908942E|aerA]) [pubmed|38470260]
very strong constitutive expression if [protein|24A86A4846187E6026A2B7645DC4B7C70908942E|aerA] acquires mutations under selective pressure by toxic amino acids [pubmed|38470260]
regulatory mechanism
[protein|24A86A4846187E6026A2B7645DC4B7C70908942E|aerA]: transcription activation, [pubmed|38470260], in [regulon|protein:24A86A4846187E6026A2B7645DC4B7C70908942E|aerA regulon]
Biological materials
Mutant
GP3955 ([gene|3BD05DE3ED5B293980F47EB621C54C7339F78DC7|aexA]::''cat''), available in [wiki|Jörg Stülke]'s lab [Pubmed|38470260]
MGNA-C080 (ydeD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2078 NBRP B. subtilis, Japan]
BKE05160 ([gene|3BD05DE3ED5B293980F47EB621C54C7339F78DC7|aexA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACATGACTCCTCACTAA, downstream forward: _UP4_TAGGGGCTTTTTTTGCTTAC
BKK05160 ([gene|3BD05DE3ED5B293980F47EB621C54C7339F78DC7|aexA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACATGACTCCTCACTAA, downstream forward: _UP4_TAGGGGCTTTTTTTGCTTAC
Expression vectors
pGP3727: expression of [gene|3BD05DE3ED5B293980F47EB621C54C7339F78DC7|aexA] (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab [pubmed|38470260]
lacZ fusion
GP4132, cat, (based on [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab [pubmed|38470260]
References
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Time of last update: 2024-12-21 02:51:23
Author of last update: Jstuelk