phoB
168
alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase
locus
BSU_05740
Molecular weight
50.36 kDa
pI
5.89
function
aquisition of phosphate upon phosphate starvation, ether lipid synthesis
product
alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase
essential
no
ec
3.1.3.1
synonyms
phoB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1785 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
620,097 621,485
The protein
Catalyzed reaction/ biological activity
phosphate monoester + H2O --> alcohol + phosphate (according to UniProt)
Protein family
alkaline phosphatase family (with [protein|E0430C16C41440633647976A0F9183E24268E198|phoA], according to UniProt)
Structure
[PDB|3A52] (from ''Shewanella sp.'', 41% identity) [Pubmed|20057143]
[AF|P19405]
Modification
phosphorylated on ser/ thr/ tyr [Pubmed|16493705]
Paralogous protein(s)
[protein|E0430C16C41440633647976A0F9183E24268E198|phoA]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]-[gene|66D3E978E3DBA0410CA5597B5B860DF27C97335A|ydhF]
description
[Pubmed|10913081]
regulation
expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|9335276,10913081,16030210]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|9335276,10913081,16030210], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,16030210], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
1A767 ( ''phoB''::''cat''), [Pubmed|9426145], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A767&Search=1A767 BGSC]
BKE05740 ([gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGGATAACCTCCTAAA, downstream forward: _UP4_TAAAAGAATACAAGGTGTCC
BKK05740 ([gene|3B95AB3021280215B6B487A348D78FB5797AEEA6|phoB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGGATAACCTCCTAAA, downstream forward: _UP4_TAAAAGAATACAAGGTGTCC
References
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Time of last update: 2024-12-20 20:35:49
Author of last update: Melvin.boenninger