pspA
168
phage shock protein A homolog, protects the membrane against membrane-targeting antibiotics
locus
BSU_06180
Molecular weight
24.99 kDa
pI
5.78
function
protection of the membrane
product
phage shock protein A homolog
essential
no
synonyms
pspA, ydjF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1842 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
671,245 671,928
Phenotypes of a mutant
more sensitive to nisin [Pubmed|23980836]
The protein
Protein family
PspA/IM30 family (with [protein|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH], according to UniProt)
Structure
[PDB|4WHE] (from E. coli, 34% identity, corresponds to aa 1 ... 133) [pubmed|26235546]
[AF|P54617]
Paralogous protein(s)
[protein|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]
[wiki|Localization]
cytoplasma (according to UniProt)
localizes to the membrane under stress conditions (localization depends of [protein|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]) [pubmed|34777311]
Expression and Regulation
Operons
genes
[gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]-[gene|F93381205C61665820D034A384BD60410AAE84C1|ydjG]-[gene|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH]-[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]
description
[Pubmed|11454200]
regulation
induced by alkaline shock ([protein|search|SigW]) [Pubmed|11454200]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18840696,20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11454200], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
MGNA-C430 (yqgI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2428 NBRP B. subtilis, Japan]
BKE06180 ([gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGTTCCTCCTAA, downstream forward: _UP4_TAATACATAGGAGGCCGCAG
BKK06180 ([gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGTTCCTCCTAA, downstream forward: _UP4_TAATACATAGGAGGCCGCAG
References
Reviews
Original Publications
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Time of last update: 2024-12-29 21:41:32
Author of last update: Jstuelk