odhA
168
2-oxoglutarate dehydrogenase (E1 subunit)
locus
BSU_19370
Molecular weight
105.54 kDa
pI
5.87
function
TCA cycle
product
2-oxoglutarate dehydrogenase (E1 subunit)
essential
no
ec
1.2.4.2
synonyms
odhA, citK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0567 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,108,774 2,111,608
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase]-(R)-N6-lipoyl-L-lysine + H+ --> [dihydrolipoyllysine-residue succinyltransferase]-(R)-N6-(S8-succinyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
Protein family
alpha-ketoglutarate dehydrogenase family (single member, according to UniProt)
[wiki|Cofactors]
TPP [pubmed|35988322]
Structure
[PDB|2JGD] (from ''Escherichia coli'', 38% identity, 55% similarity) [Pubmed|17367808]
[AF|P23129]
Modification
phosphorylated on Arg-66 and Arg-621 [Pubmed|22517742]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/2-Oxoglutarate_Dehydrogenase Proteopedia]
Expression and Regulation
Operons
genes
[gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB]
description
[Pubmed|11976317]
regulation
repressed by glucose (2.4-fold) ([protein|search|CcpA]) [Pubmed|12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1508153], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP671 (spc), GP684 (cat), available in [wiki|Jörg Stülke]'s lab
GP1274 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA])::''cat'', available in [wiki|Jörg Stülke]'s lab
GP1276 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''cat'', available in [wiki|Jörg Stülke]'s lab
GP2183 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA])::''ermC'', available in [wiki|Jörg Stülke]'s lab [pubmed|28679749]
GP2332 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jörg Stülke]'s lab
GP2334 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jörg Stülke]'s lab
BKE19370 ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATATTACCCCCAA, downstream forward: _UP4_AAAAACTAAGGGGGAAATGA
BKK19370 ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGAATATTACCCCCAA, downstream forward: _UP4_AAAAACTAAGGGGGAAATGA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP3106 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-20 21:38:45
Author of last update: Jstuelk