pbpA
168
class B penicillin-binding protein PBP 2A, required for [wiki|cell wall synthesis] during cell elongation, works together with [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] in the [wiki|elongasome] for lateral [wiki|cell wall synthesis] and control of cell diameter
locus
BSU_25000
Molecular weight
79.96 kDa
pI
9.57
function
formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
product
penicillin-binding protein PBP 2A
essential
no
synonyms
pbpA, yqgF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,581,771 2,583,921
Phenotypes of a mutant
a [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA] [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH] double mutant is not viable [pubmed|36993441]
The protein
Catalyzed reaction/ biological activity
required for [wiki|cell wall synthesis] during cell elongation [Pubmed|12896990]
driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] dynamics [Pubmed|21636744,21636745]
transpeptidase activity [pubmed|33709487]
Protein family
[wiki|transpeptidase family] (according to UniProt)
Structure
[PDB|3VSK] (from Staphylococcus aureus, 45% identity) [pubmed|22846910]
[AF|P54488]
Paralogous protein(s)
[protein|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]
Expression and Regulation
Operons
genes
[gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]
description
[Pubmed|21815947]
additional information
the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]
BKE25000 ([gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
BKK25000 ([gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jeff Errington] lab
GFP fusion
3103 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::pSG5043 (cat Pxyl-gfpa-[gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]1-804) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
labs
[wiki|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
Original Publications
Page visits: 6222
Time of last update: 2024-12-29 22:12:22
Author of last update: Jstuelk