ycxD
168
similar to transcriptional regulator ([wiki|MocR/ GabR family])
locus
BSU_03560
Molecular weight
50.59 kDa
pI
8.57
function
unknown
product
unknown
essential
no
synonyms
ycxD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
406,131 407,465
The protein
Protein family
[wiki|MocR/ GabR family] [Pubmed|22020104]
[wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Domains]
N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
[wiki|HTH gntR-type domain] (aa 1-69) (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR], 27% identity) [pubmed|24127574]
[AF|Q08792]
Biological materials
Mutant
BKE03560 ([gene|3A756A845ADA88DA569FA9A1B484DA43A28C6F4D|ycxD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTTCTCCCCCTGTTT, downstream forward: _UP4_GGTGTCAAGCTGTTGATGAG
BKK03560 ([gene|3A756A845ADA88DA569FA9A1B484DA43A28C6F4D|ycxD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCCTTCTCCCCCTGTTT, downstream forward: _UP4_GGTGTCAAGCTGTTGATGAG
Expression vectors
pGP3930 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
References
Page visits: 1971
Time of last update: 2024-11-22 23:57:54
Author of last update: Dennis.wicke