efeB
168
elemental iron uptake system, A-type dye-decolorizing peroxidase, converts ferrous iron (Fe(II) to ferric iron (FeIII)) for uptake by [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[protein|3BED98E311EF380AE77327C7F0A83254805089C0|efeU], peroxide detoxification under microaerobic conditions
locus
BSU_38260
Molecular weight
45.53 kDa
pI
8.64
function
ferrous iron conversion
product
heme peroxidase in elemental iron uptake
essential
no
ec
1.11.1.-
synonyms
efeB, efeN, ipa-29d, ywbN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2837 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,926,682 3,927,932
Phenotypes of a mutant
poor growth with Fe(II) under microaerobic conditions [pubmed|23764491]
The protein
Catalyzed reaction/ biological activity
oxidizes ferrous iron to ferric iron for uptake by [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[protein|3BED98E311EF380AE77327C7F0A83254805089C0|efeU] [Pubmed|23764491]
eliminates reactive oxygen species that accumulate in the presence of ferrous iron [Pubmed|23764491]
peroxide detoxification under microaerobic conditions [Pubmed|23764491]
Protein family
DyP-type peroxidase family (single member, according to UniProt)
[wiki|Cofactors]
protoheme IX [Pubmed|23764491]
Structure
[PDB|6KMN] [pubmed|32971035]
[PDB|7PKX] [pubmed|34639208]
[PDB|7E5Q] (aa 56 ... 416) [pubmed|36345957]
[AF|P39597]
Effectors of protein activity
activity is increased in the presence of [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO] due to the displacement of ferric iron [Pubmed|23764491]
[wiki|Localization]
extracellular, secreted by the [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY] complex [Pubmed|15554971]
[protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]-dependent export of [protein|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB] requires a functional [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|wprA] [Pubmed|23180473]
forms a membrane-bound complex with [protein|3BED98E311EF380AE77327C7F0A83254805089C0|efeU] and [protein|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO] for iron uptake [Pubmed|23180473,23764491]
attached to the membrane via [protein|3BED98E311EF380AE77327C7F0A83254805089C0|efeU] [Pubmed|23764491]
Expression and Regulation
Operons
genes
[gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
description
[Pubmed|9353933]
regulation
immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23764491], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]-[gene|65FC8F21D8927C231B55E8693BD29F052A830AF6|ywbO]
description
[Pubmed|18179421]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9683469], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
Biological materials
Mutant
MGNA-B228 (ywbN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1227 NBRP B. subtilis, Japan]
BKE38260 ([gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTTACAAAACTCCT, downstream forward: _UP4_TAAAAGAAGCCTTTACAGGC
BKK38260 ([gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTTACAAAACTCCT, downstream forward: _UP4_TAAAAGAAGCCTTTACAGGC
labs
[wiki|Jan Maarten van Dijl], Groningen, Netherlands
References
Reviews
Original Publications
Page visits: 6337
Time of last update: 2024-12-21 06:03:41
Author of last update: Jstuelk