spsC
168
aminotransferase, legionaminic acid synthesis
locus
BSU_37890
Molecular weight
43.03 kDa
pI
5.55
function
legionaminic acid synthesis
product
aminotransferase
essential
no
synonyms
spsC, ipa-65d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0399 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,889,732 3,890,901
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
less hydrophobic spore surface [pubmed|32817102]
The protein
Catalyzed reaction/ biological activity
legionaminic acid synthesis [pubmed|32817102]
Protein family
DegT/DnrJ/EryC1 family (with [protein|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN] and [protein|2F850E2DFA6287DB3890243803E06FDE7ACDB10E|ntdA], according to UniProt)
[wiki|Cofactors]
PLP [pubmed|12429098]
Structure
[PDB|1MDX] (ArnB from Salomonella typhimurium, 44% identity) [pubmed|12429098]
[AF|P39623]
[wiki|Localization]
binds the outer surface of the forming spore [Pubmed|14602648]
Expression and Regulation
Operons
genes
[gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
description
[Pubmed|9353933]
regulation
expressed during [wiki|sporulation] in the mother cell ([wiki|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|25239894,15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|26577401], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
CS218 (''[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s labs) [Pubmed|27197833]
BKE37890 ([gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTGCGCTCCTATC, downstream forward: _UP4_GATATCGTAAAAGGGGCTGA
BKK37890 ([gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTGCGCTCCTATC, downstream forward: _UP4_GATATCGTAAAAGGGGCTGA
References
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Time of last update: 2024-12-29 08:19:08
Author of last update: Jstuelk