msrA
168
peptide methionine sulfoxide reductase
locus
BSU_21690
Molecular weight
20.04 kDa
pI
5.71
function
regeneration of methionine and restoration of protein function after oxidative damage
product
peptide methionine sulfoxide reductase
essential
no
ec
1.8.4.11
synonyms
msrA, yppP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0225 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,287,529 2,288,062
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + H2O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein](according to UniProt)
[thioredoxin]-disulfide + H2O + L-methionine --> [thioredoxin]-dithiol + L-methionine (S)-S-oxide (according to UniProt)
Protein family
MsrA Met sulfoxide reductase family (single member, according to UniProt)
Structure
[PDB|2GT3] (from E. coli, 45% identity) [pubmed|17157315]
[AF|P54154]
Expression and Regulation
Operons
genes
[gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]
description
[Pubmed|18662407]
regulation
induced by oxidative stress ([protein|search|Spx])
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, oxidized [protein|2C6386E9A63F410558D168798D077DF91590F454|spx] binds [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA] and enhances [protein|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[protein|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB] operon transcription [Pubmed|18662407], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18662407,17289755], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A894 (yppP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/894 NBRP B. subtilis, Japan]
BKE21690 ([gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT, downstream forward: _UP4_TAAATGGCGTACAATAAAGA
BKK21690 ([gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT, downstream forward: _UP4_TAAATGGCGTACAATAAAGA
References
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Time of last update: 2025-01-01 10:45:28
Author of last update: Melvin.boenninger