msrA

msrA
168

peptide methionine sulfoxide reductase

Locus
BSU_21690
Molecular weight
20.04 kDa
Isoelectric point
5.71
Protein length
177 aaSequenceBlast
Gene length
534 bpSequenceBlast
Function
regeneration of methionine and restoration of protein function after oxidative damage
Product
peptide methionine sulfoxide reductase
Essential
no
E.C.
1.8.4.11
Synonyms
msrA, yppP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0225 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,287,529  2,288,062
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + H2O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein](according to UniProt)
[thioredoxin]-disulfide + H2O + L-methionine --> [thioredoxin]-dithiol + L-methionine (S)-S-oxide (according to UniProt)
Protein family
MsrA Met sulfoxide reductase family (single member, according to UniProt)
Structure
2GT3 (PDB) (from E. coli, 45% identity) PubMed
P54154 (AlphaFold)
Expression and Regulation
Operons
Genes
msrA-msrB
Description
PubMed
Regulation
induced by oxidative stress (Spx)
Regulatory mechanism
Spx: activation, oxidized Spx binds RpoA and enhances MsrA-MsrB operon transcription PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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msrA→msrB

2025-04-01 05:29:42

ghost

85

260530b389542edd9065dae32bb081f8ff5b9af0

31A91806DA37296DA2E76252C321BA878BFF3D0A

Biological materials
Mutant
MGNA-A894 (yppP::erm), available at the NBRP B. subtilis, Japan
BKE21690 (msrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT,  downstream forward: _UP4_TAAATGGCGTACAATAAAGA
BKK21690 (msrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTGGTGCCTCCTTTGT,  downstream forward: _UP4_TAAATGGCGTACAATAAAGA
References
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389245FDE13226D65DF117E28B5DA346DC856860

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Time of last update: 2025-04-18 00:40:24

Author of last update: Melvin.boenninger