minD

minD
168

[wiki|cell division] inhibitor (septum placement), part of the Min system (with Z ring placement), required for the disassembly of polar [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|Z rings] after [wiki|cell division]

locus
BSU_27990
Molecular weight
29.26 kDa
pI
4.98
Protein length
Gene length
function
septum placement
product
[wiki|cell division] inhibitor
essential
no
synonyms
minD, divIVB1

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2894 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,857,776  2,858,582
Phenotypes of a mutant
produces minicells [pubmed|33849976,32184253]
increased cell length [pubmed|33849976]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD] mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
severe growth defect of a [gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant at increased temperature (37°C or above) due to a maturation defect of the Z-ring for [wiki|cell division], this can be suppressed by any mutation affecting the [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-[protein|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH] proteolytic system due to [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]-mediated increased expression of [protein|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] [pubmed|34181483]
The protein
Catalyzed reaction/ biological activity
The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle  [Pubmed|20352045]
required for oriC placement during spore development [Pubmed|27059541]
Protein family
ParA family (with [protein|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|flhG] and [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA], according to UniProt)
Structure
[PDB|4V03] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|25500731]
[AF|Q01464]
[wiki|Localization]
polar/septal at the cell membrane [Pubmed|20566861]
membrane binding/polar localization depends on the proton motive force [Pubmed|20566861]
Additional information
about 3,500 molecules per cell [pubmed|33849976]
Expression and Regulation
Operons
genes
[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|26091431], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC] [Pubmed|8459776], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Open in new tab

[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]→[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]

2024-10-06 07:53:02

ghost

124

df866addcf0a1b269336e8d5bd5fbd5f95fab5a3

37AF85C74BFE779D7BD018F30628D4588CACE9D3

genes
[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
Open in new tab

[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]→[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]

2024-08-29 16:53:56

ghost

79

3cf77b66614658eaae6b7fe62e467485f48b1db2

5296B8543F3BF57CD786DD7DA18A4244A4551659

genes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]→[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]

2024-10-06 08:09:37

ghost

117

5cb36570cb9eb74ac55107ce2bd7f07e7d2d5c0b

EE31E7A5D1B0C8BDC56B004AA68A1C721D4DA9CC

Biological materials
Mutant
BKE27990 ([gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGTTATTACGATAGCCTCAC,  downstream forward: _UP4_TAATGTGATAGAATCAAAGA
BKK27990 ([gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGTTATTACGATAGCCTCAC,  downstream forward: _UP4_TAATGTGATAGAATCAAAGA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
26286503,19884039,24367361,28697666,35638784
Original Publications
8459776,19019154,11886553,9808628,1400224,12368265,10411726,15317782,12492861,20352045,11886553,20566861,18179421,21821766,22457634,21926231,24097947,25374563,25500731,22383849,26091431,27059541,28674273,30092000,32184253,33686188,33849976,34181483,35495694

37DAD42A391E2FC506225EAF91B8F21629A401DF

Page visits: 5136

Time of last update: 2024-10-05 10:38:43

Author of last update: Jstuelk