yrvM

yrvM
168

2-methylthio-N6-threonylcarbamoyladenosine cyclase, catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A )

locus
BSU_27540
Molecular weight
28.02 kDa
pI
8.19
Protein length
254 aaSequenceBlast
Gene length
765 bpSequenceBlast
function
tRNA modification
product
2-methylthio-N6-threonylcarbamoyladenosine cyclase
essential
no
synonyms
tcdA, yrvM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1179 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,813,643  2,814,407
The protein
Catalyzed reaction/ biological activity
catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form  2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A )  [Pubmed|27913733,23242255]
Protein family
HesA/MoeB/ThiF family (with [protein|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB] and [protein|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF], according to UniProt)
Structure
[PDB|4D7A] (from ''E. coli'', 40% identity) [Pubmed|25897750]
[AF|O32037]
Expression and Regulation
Operons
genes
[gene|A0E4AC7E74B5374198D047589629DA96A681CAF4|bsrA]-[gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]
description
[Pubmed|22383849]
Open in new tab

[gene|A0E4AC7E74B5374198D047589629DA96A681CAF4|bsrA]→[gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]

2024-10-25 17:23:48

Jstuelk

64

a63daa76bafc2c8aaac1ca0a548c3453fcedd97c

B9F233760104648EEDB610E25C8C277EE8818F31

Biological materials
Mutant
MGNA-A826 (yrvM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/826 NBRP B. subtilis, Japan]
BKE27540 ([gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAACCTCACTCCACATT,  downstream forward: _UP4_TAATGCATAAAAACAGCCTT
BKK27540 ([gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAACCTCACTCCACATT,  downstream forward: _UP4_TAATGCATAAAAACAGCCTT
References
23242255,27913733,25897750

37DA3A036937660AA750065B46B581D911D73D00

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Time of last update: 2024-12-21 18:03:20

Author of last update: Melvin.boenninger