yrvM
168
2-methylthio-N6-threonylcarbamoyladenosine cyclase, catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A )
locus
BSU_27540
Molecular weight
28.02 kDa
pI
8.19
function
tRNA modification
product
2-methylthio-N6-threonylcarbamoyladenosine cyclase
essential
no
synonyms
tcdA, yrvM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1179 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,813,643 2,814,407
The protein
Catalyzed reaction/ biological activity
catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A ) [Pubmed|27913733,23242255]
Protein family
HesA/MoeB/ThiF family (with [protein|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB] and [protein|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF], according to UniProt)
Structure
[PDB|4D7A] (from ''E. coli'', 40% identity) [Pubmed|25897750]
[AF|O32037]
Expression and Regulation
Operons
genes
[gene|A0E4AC7E74B5374198D047589629DA96A681CAF4|bsrA]-[gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A826 (yrvM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/826 NBRP B. subtilis, Japan]
BKE27540 ([gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAACCTCACTCCACATT, downstream forward: _UP4_TAATGCATAAAAACAGCCTT
BKK27540 ([gene|37DA3A036937660AA750065B46B581D911D73D00|yrvM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAACCTCACTCCACATT, downstream forward: _UP4_TAATGCATAAAAACAGCCTT
References
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Time of last update: 2024-12-21 18:03:20
Author of last update: Melvin.boenninger