hpf
168
general stress protein, required for ribosome dimerization in the stationary phase, protects essential ribosomal proteins ([protein|F1969E4C7BAAF70BCBE570F58350C12A3E417539|S2] and [protein|E7F4E01A6005C8469EAC64E8DFED96D0F29791A9|S3])
locus
BSU_35310
Molecular weight
21.83 kDa
pI
5.18
function
protection of essential ribosomal proteins ([protein|F1969E4C7BAAF70BCBE570F58350C12A3E417539|S2] and [protein|E7F4E01A6005C8469EAC64E8DFED96D0F29791A9|S3])
product
ribosome hibernation promoting factor
essential
no
synonyms
hpf, yviI, yvyD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1544 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,631,003 3,631,572
Phenotypes of a mutant
the mutant is cold-sensitive
delayed recovery from stationary phase and delayed [wiki|germination] [Pubmed|26743942]
The protein
Catalyzed reaction/ biological activity
[protein|search|Hpf ]binds the 30S subunits of hibernating [wiki|ribosome]s, dimerization of [protein|search|Hpf ]results in 100S [wiki|ribosome ]dimer formation [pubmed|28468753,26743942]
Protein family
HPF/YfiA ribosome-associated protein family (single member, according to UniProt)
Structure
[PDB|5NJT] (N-terminal domain (aa 1 ... 104) of Hpf as part of the 100S [wiki|ribosome]) [pubmed|28468753]
[PDB|8UU4] (complex with the ribosome, from ''Listeria monocytogenes'', 60.3% identity) [pubmed|38407413]
[AF|P28368]
Modification
phosphorylated on Arg-6 [Pubmed|22517742]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
[protein|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf] is degraded in the forespore using [protein|7CFDFB42AF816FF5766086E537472008E6CB7E85|mdfA] as the adaptor protein [pubmed|39651166]
Expression and Regulation
Operons
genes
[gene|4DBF4635B5C5A7541E8F97440D139DAA02547B99|yvyC]-[gene|C6012DEA301DF5B2DDD7E673A736BD3E5163F209|fliD]-[gene|4882FAD988FEDD0E72E4CAFB10C8DE61DDAC23B0|fliS]-[gene|4DE156D248FAF7DE3ED9AC35F006B5319A3C1BDA|fliT]-[gene|A6E7212D159F076F5D26F9C02F340B40C3667623|smiA]-[gene|06FEA5F205B40DD5CF8D9F1328E940D4543F4741|S1350]-[gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]
description
[pubmed|22383849]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
genes
[gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]
description
[Pubmed|9852014]
regulation
expressed in the stationary phase [Pubmed|26743942]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|25666134], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|9852014,15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|9852014], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
BKE35310 ([gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAGAACGCCTCCCTT, downstream forward: _UP4_TAATGAAGAGAAGCCTTCCG
BKK35310 ([gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAGAACGCCTCCCTT, downstream forward: _UP4_TAATGAAGAGAAGCCTTCCG
GP2598 ([gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]::tet comIQ12L) (in DK1042) available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-22 01:15:29
Author of last update: Jstuelk