bdhA
168
acetoine/ butanediol dehydrogenase
locus
BSU_06240
Molecular weight
37.19 kDa
pI
4.8
function
overflow metabolism, fermentation
product
acetoine/ butanediol dehydrogenase
essential
no
ec
1.1.1.76
synonyms
bdhA, ydjL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
677,911 678,951
The protein
Catalyzed reaction/ biological activity
formation of butanediol from acetoin [Pubmed|18820069]
(R,R)-butane-2,3-diol + NAD+ --> (R)-acetoin + H+ + NADH (according to UniProt)
Protein family
[wiki|zinc-containing alcohol dehydrogenase family] (according to UniProt)
[wiki|Cofactors]
NAD+ (according to UniProt)
Structure
[PDB|6IE0]
[AF|O34788]
Modification
phosphorylated on Arg-13 [Pubmed|22517742]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|37A9CD057DC311397B9A5757F6ED7AE6998F6D5D|bdhA]
description
[Pubmed|22383849]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
Biological materials
Mutant
MGNA-C219 (ydjL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2217 NBRP B. subtilis, Japan]
BKE06240 ([gene|37A9CD057DC311397B9A5757F6ED7AE6998F6D5D|bdhA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA
BKK06240 ([gene|37A9CD057DC311397B9A5757F6ED7AE6998F6D5D|bdhA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGATTACCACTCCTATA, downstream forward: _UP4_TAATTTGAAACCAAAAAGAA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab
References
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Time of last update: 2024-10-14 14:41:13
Author of last update: Jstuelk