infB
168
translation initiation factor IF-2, ribosome-dependent GTPase interacting with 30 ribosome subunits, ensures correct binding of initiator tRNAs
locus
BSU_16630
Molecular weight
78.35 kDa
pI
5.19
function
translation
product
initiation factor IF-2
essential
yes
synonyms
infB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0532 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,734,009 1,736,159
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ribosome-dependent GTPase interacting with 30 ribosome subunits, ensures correct binding of initiator tRNAs [pubmed|15755955,26259514]
Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients [PubMed|16968770]
Protein family
[wiki|TRAFAC class translation factor GTPase superfamily] (according to UniProt)
[wiki|Classic translation factor GTPase family] (according to UniProt)
[wiki|Domains]
Nucleotide-binding domain that competitively binds GTP or ppGpp [Pubmed|16968770]
tr-type G domain (aa 217-386) (according to UniProt)
Structure
[PDB|1D1N] (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), [PDB|1Z9B] (C1-subdomain, Geobacillus stearothermophilus)
[AF|P17889]
Effectors of protein activity
GTP and ppGpp [Pubmed|16968770]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|9F6C447900410BF64409E5C41A11C76968DADAFA|ylxS]-[gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]-[gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]-[gene|7BFA65857F69855DBB3BB64D93B4ED2F09DC705B|rplGA]-[gene|378B77412D0DEA220A97DEC246410509D9B74D46|infB]-[gene|24835CE6CD2E1F183DB9121F29A63101718B5DF9|ylxP]-[gene|B3119A40393A288EF34FC3E9839B6E40AC6A6EA0|rbfA]
description
[Pubmed|8491709]
regulation
autoregulation [wiki|NusA] [pubmed|27571753]
induced by glucose [pubmed|30355672]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
[protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]: attenuation, [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA] stimulates termination [Pubmed|27571753], in [regulon|protein:8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8491709], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528,26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
References
Reviews
Original Publications
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Time of last update: 2024-12-29 17:01:03
Author of last update: Jstuelk