prs
168
phosphoribosylpyrophosphate synthetase, universally conserved protein
locus
BSU_00510
Molecular weight
34.71 kDa
pI
5.89
function
phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
product
phosphoribosylpyrophosphate synthetase
essential
yes
ec
2.7.6.1
synonyms
prs
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0462 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
57,745 58,698
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + D-ribose 5-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + AMP + H+ (according to UniProt)
Protein family
Ribose-phosphate pyrophosphokinase family (single member, according to UniProt)
Structure
[PDB|1IBS]
[PDB|1DKR]
[PDB|1DKU]
[AF|P14193]
Effectors of protein activity
subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
[wiki|universally conserved protein]
Expression and Regulation
Operons
genes
[gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
description
[Pubmed|22383849]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|8522540], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Expression vectors
N-terminal Strep-tag, expression in ''E. coli'', in [wiki|pGP172]: pGP2541, available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-10-03 12:49:33
Author of last update: Melvin.boenninger