guaC
168
GMP reductase
locus
BSU_32130
Molecular weight
35.66 kDa
pI
6.08
function
purine salvage and interconversion
product
GMP reductase
essential
no
ec
1.7.1.7
synonyms
guaC, yumD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0516 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,303,042 3,304,022
The protein
Catalyzed reaction/ biological activity
Inosine 5'-phosphate + NH3 + NADP+ --> guanosine 5'-phosphate + NADPH (according to UniProt)
Protein family
IMPDH/GMPR family (together with [protein|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB]) (according to UniProt)
Structure
[PDB|1YPF] (from ''Bacillus anthracis'', 84% identity, 91% similarity)
[AF|O05269]
Expression and Regulation
Operons
genes
[gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]
description
[Pubmed|21815947]
regulation
activated during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|12618455]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
additional information
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 3 to 20 min) [PubMed|21815947]
Biological materials
Mutant
MGNA-B573 (yumD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1572 NBRP B. subtilis, Japan]
BKE32130 ([gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA, downstream forward: _UP4_TAATCATAAAAAAACGCCAA
BKK32130 ([gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA, downstream forward: _UP4_TAATCATAAAAAAACGCCAA
References
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Time of last update: 2024-12-22 05:23:52
Author of last update: Jstuelk