guaC

guaC
168

GMP reductase

locus
BSU_32130
Molecular weight
35.66 kDa
pI
6.08
Protein length
Gene length
function
purine salvage and interconversion
product
GMP reductase
essential
no
ec
1.7.1.7
synonyms
guaC, yumD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0516 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,303,042  3,304,022
The protein
Catalyzed reaction/ biological activity
Inosine 5'-phosphate + NH3 + NADP+ --> guanosine 5'-phosphate + NADPH (according to UniProt)
Protein family
IMPDH/GMPR family (together with [protein|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB]) (according to UniProt)
Structure
[PDB|1YPF] (from ''Bacillus anthracis'', 84% identity, 91% similarity)
[AF|O05269]
Expression and Regulation
Operons
genes
[gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]
description
[Pubmed|21815947]
regulation
activated during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|12618455]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: activation, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
additional information
the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 3 to 20 min) [PubMed|21815947]
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[gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]

2024-12-16 20:55:12

Jstuelk

52

71f13bdff20288155b7175c51fd38331548ac54d

BF214831E80A166BF10E23C913865483F782FDA3

Biological materials
Mutant
MGNA-B573 (yumD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1572 NBRP B. subtilis, Japan]
BKE32130 ([gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA,  downstream forward: _UP4_TAATCATAAAAAAACGCCAA
BKK32130 ([gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA,  downstream forward: _UP4_TAATCATAAAAAAACGCCAA
References
11591660,2536750,12618455,12850135,21815947

350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665

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Time of last update: 2024-12-22 05:23:52

Author of last update: Jstuelk