mgsA
168
methylglyoxal synthase
locus
BSU_22480
Molecular weight
14.99 kDa
pI
4.92
function
bypass of glycolysis
product
methylglyoxal synthase
essential
no
ec
4.2.3.3
synonyms
mgsA, ypjF, jojF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1803 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,358,911 2,359,324
The protein
Catalyzed reaction/ biological activity
dihydroxyacetone phosphate --> methylglyoxal + phosphate (according to UniProt)
Protein family
methylglyoxal synthase family (single member, according to UniProt)
[wiki|Domains]
MGS-like domain (aa 1-137) (according to UniProt)
Structure
[PDB|6F2C] [pubmed|29514981]
[AF|P42980]
Effectors of protein activity
non-phosphorylated [protein|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh] interacts with [protein|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA] to inhibit its activity [Pubmed|29514981,21992469]
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A440 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/440 NBRP B. subtilis, Japan]
GP67 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::''tet'') [Pubmed|21992469], available in [wiki|Jörg Stülke]'s lab
GP84 (''mgsA''::''pX2''(''cat'')), available in [wiki|Jörg Stülke]'s lab
BKE22480 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
BKK22480 ([gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTTTCATTGTTTATCCCC, downstream forward: _UP4_GACCTTCTTCGGGGAGAAGA
Expression vectors
pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP1459]), available in [wiki|Jörg Stülke]'s lab
pGP2505 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab [Pubmed|21992469]
FLAG-tag construct
GP86 (spc, based on [wiki|pGP1331]) [Pubmed|21992469], available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Boris Görke], University of Vienna, Austria
[wiki|Jörg Stülke], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
References
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Time of last update: 2024-10-13 15:53:23
Author of last update: Jstuelk