mtnB

mtnB
168

methylthioribulose-1-phosphate dehydratase

Locus
BSU_13610
Molecular weight
23.34 kDa
Isoelectric point
5.69
Protein length
209 aaSequenceBlast
Gene length
630 bpSequenceBlast
Function
methionine salvage
Product
methylthioribulose-1-phosphate dehydratase
Essential
no
E.C.
4.2.1.109
Synonyms
mtnB, ykrY
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0235 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,428,940  1,429,569
The protein
Catalyzed reaction/ biological activity
5-methylsulfanyl-D-ribulose 1-phosphate --> 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O (according to UniProt)
Protein family
aldolase class II family (with AraD, according to UniProt)
Structure
2IRP (PDB) (from Aquifex aeolicus, 40% identity)
O31668 (AlphaFold)
Expression and Regulation
Operons
Genes
mtnW-mtnX-mtnB-mtnD
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mtnW→mtnD

2025-03-15 03:32:16

Jstuelk

82

a2b13ba83890892f9d8595d5e452be227045e93c

6416B0A5CDDC144986D3159AFC5B04D4507F2AE1

Biological materials
Mutant
MGNA-B322 (ykrY::erm), available at the NBRP B. subtilis, Japan
BKE13610 (mtnB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCTCTCTCTGCCAATTCCC,  downstream forward: _UP4_GTTAAATAAAAGGAGGAATT
BKK13610 (mtnB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCTCTCTCTGCCAATTCCC,  downstream forward: _UP4_GTTAAATAAAAGGAGGAATT
References
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Page visits: 4145

Time of last update: 2025-03-15 07:35:17

Author of last update: Melvin.boenninger