galE
168
UDP glucose 4-epimerase
locus
BSU_38860
Molecular weight
36.85 kDa
pI
4.78
function
galactose utilization
product
UDP glucose 4-epimerase
essential
no
ec
5.1.3.2
synonyms
galE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1087 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,989,948 3,990,967
Phenotypes of a mutant
smooth colonies on MsGG medium, no [wiki|biofilm formation] in the absence of externally provided galactose [Pubmed|22893383,22113911]
the mutant is sensitive to exogenous galactose due to the accumulation of toxic UDP-galactose, this sensitivity can be suppressed by the inactivation of ''[gene|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK]'' (no formation of the toxic UDP-galactose) or of ''[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]'' (constitutive expression of the ''[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]-[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]-[gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]-[gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]-[gene|DB47BA2A5E3BED51DD8630E7D321C099D74B46CF|epsF]-[gene|C4D81976AAE888C4FE89A2EE5CF9A763CAD645FF|epsG]-[gene|BD843389F95BFB905071547AB243413A617F12A8|epsH]-[gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]-[gene|5272E807B6B98541EA155AA9748320E25767F096|epsJ]-[gene|66076952E512D53FC1FC62455A86ACFA21AE120D|epsK]-[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]-[gene|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM]-[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]'' results in detoxification of UDP-galactose) [Pubmed|22893383]
The protein
Catalyzed reaction/ biological activity
UDP-α-D-galactose --> UDP-α-D-glucose (according to UniProt)
Protein family
[wiki|NAD(P)-dependent epimerase/dehydratase family] (according to UniProt)
Structure
[PDB|1LRL] (from ''Escherichia coli Y299C mutant'', 57% identity, 71% similarity)\t[Pubmed|12019271]
[AF|P55180]
Expression and Regulation
Operons
genes
[gene|314223775ECB9E969F4F898584FC4E5379E86C7F|galE]
description
[Pubmed|10746760]
regulation
strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9555917], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|28DA9F2012E19D36879F9799BBEF153FDEE7659B|yxkA]-[gene|314223775ECB9E969F4F898584FC4E5379E86C7F|galE]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B736 (galE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1735 NBRP B. subtilis, Japan]
BKE38860 ([gene|314223775ECB9E969F4F898584FC4E5379E86C7F|galE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTAAACCCTCCTAA, downstream forward: _UP4_TAAGAATGGAGGCCTTCTCA
BKK38860 ([gene|314223775ECB9E969F4F898584FC4E5379E86C7F|galE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTAAACCCTCCTAA, downstream forward: _UP4_TAAGAATGGAGGCCTTCTCA
References
Page visits: 4705
Time of last update: 2025-04-05 16:21:41
Author of last update: Jstuelk