tsaD

tsaD
168

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein

locus
BSU_05940
Molecular weight
36.69 kDa
pI
5.02
Protein length
346 aaSequenceBlast
Gene length
1041 bpSequenceBlast
function
tRNA modification
product
tRNA modification enzyme
essential
yes
ec
2.3.1.234
synonyms
tsaD, ydiE, gcp
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0533 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
643,258  644,298
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]) [Pubmed|23072323,21183954]
adenosine37 in tRNA + L-threonylcarbamoyladenylate --> AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA (according to UniProt)
Protein family
KAE1 / TsaD family (with [protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB], according to UniProt)
Structure
[PDB|3ZET] (from ''Salmonella typhimurium'', 46% identity) [Pubmed|23471679]
[AF|O05518]
Modification
in vitro phosphorylated by [protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE] [pubmed|28890133]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
description
[Pubmed|22383849]
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[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]→[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]

2024-09-25 07:52:22

ghost

109

45839886739c513a86015eb2db5091e043ecd558

F160E3DB3BAB4687DBF8F609242CE813EE9E1BF6

Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Research papers
31118925,33101263
Publications on the corresponding protein from other species
21183954,19376873,20824107,21619589,21285948,21625506,23056478,23471679,28890133,29741707,33524148,32385138

30393F16CFE89E7BAD233A0BD27B77D679F7E409

Page visits: 3899

Time of last update: 2024-10-05 21:04:31

Author of last update: Dennis.wicke