tsaD
168
required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
locus
BSU_05940
Molecular weight
36.69 kDa
pI
5.02
function
tRNA modification
product
tRNA modification enzyme
essential
yes
ec
2.3.1.234
synonyms
tsaD, ydiE, gcp
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0533 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
643,258 644,298
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]) [Pubmed|23072323,21183954]
adenosine37 in tRNA + L-threonylcarbamoyladenylate --> AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA (according to UniProt)
Protein family
KAE1 / TsaD family (with [protein|6CB350C335603D5F4434679F9D740BFECA738599|tsaB], according to UniProt)
Structure
[PDB|3ZET] (from ''Salmonella typhimurium'', 46% identity) [Pubmed|23471679]
[AF|O05518]
Modification
in vitro phosphorylated by [protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE] [pubmed|28890133]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
description
[Pubmed|22383849]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Research papers
Publications on the corresponding protein from other species
Page visits: 3899
Time of last update: 2024-10-05 21:04:31
Author of last update: Dennis.wicke