oppA
168
oligopeptide [wiki|ABC transporter] (binding protein)
locus
BSU_11430
Molecular weight
61.32 kDa
pI
5.72
function
initiation of [wiki|sporulation], competence development
product
oligopeptide [wiki|ABC transporter] (binding protein)
essential
no
synonyms
oppA, spo0KA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4166 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,219,849 1,221,486
Phenotypes of a mutant
reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
The protein
Catalyzed reaction/ biological activity
required for the uptake of quorum sensing peptides such as [protein|4FA7D6E6316A6FC71C10C692D125833263894020|phrH] [Pubmed|21908671]
Protein family
[wiki|bacterial solute-binding protein 5 family] (according to UniProt)
Structure
[PDB|8ARN] (complex with SNSS peptide) [pubmed|36748525]
[PDB|8ARE] (complex with [protein|997434F9679B3A2F8D2453D0C3BAB24F5BB90922|phrE] peptide) [pubmed|36748525]
[PDB|1OLA] (from Salmonella enterica, 36% identity) [pubmed|8202710]
[AF|P24141]
Modification
phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 [Pubmed|17218307]
Paralogous protein(s)
[protein|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]
[wiki|Localization]
attached to the cell membrane (via [protein|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[protein|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]) [Pubmed|10092453]
at the outer side of the cell: extracellular (signal peptide) [Pubmed|18957862], lipid modification as retention signal [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]
description
[Pubmed|1901616]
regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, no derepression occurs, however, in a [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY] mutant, due to increased repression by [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC] [Pubmed|25966844], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|10383984], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|trpS]' and '[protein|search|oppA]' [PubMed|20525796]
Biological materials
Mutant
BP67 (spc) available in [wiki|Fabian Commichau]'s lab
1S118 ( ''oppA''::''spec''), [Pubmed|11267663], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S118&Search=1S118 BGSC]
GP2097 (D(''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]'')::''aphA3''), available in [wiki|Jörg Stülke]'s lab
BKE11430 (''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]''::''erm'', available in the BGSC, in [wiki|Fabian Commichau]'s, and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
BKE11430 ([gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGCAAACCCCCTAA, downstream forward: _UP4_TAAGGCTACGTCTGAAAATA
BKK11430 ([gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGCAAACCCCCTAA, downstream forward: _UP4_TAAGGCTACGTCTGAAAATA
References
Page visits: 10111
Time of last update: 2024-10-14 17:29:45
Author of last update: Jstuelk