trmU
168
tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
locus
BSU_27500
Molecular weight
42.20 kDa
pI
4.73
function
tRNA modification
product
tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
essential
yes
ec
2.8.1.-
synonyms
trmU, yrrA, mnmA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0482 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,809,413 2,810,528
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]) [Pubmed|25825430]
U34 in tRNA + L-cysteine + ATP --> (s2U34 in tRNA) + alanine + AMP + ppi [Pubmed|25825430]
Protein family
MnmA/TRMU family (single member, according to UniProt)
Structure
[PDB|2HMA] (from ''Streptococcus pneumoniae'', 66% identity)
[AF|O35020]
[wiki|Localization]
cytoplasm(according to UniProt)
Expression and Regulation
Operons
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
description
[Pubmed|22383849]
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
description
[Pubmed|16885442]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16885442], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
References
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Time of last update: 2024-10-09 01:54:23
Author of last update: Jstuelk