mmgF
168
methylisocitrate lyase
locus
BSU_24120
Molecular weight
32.94 kDa
pI
5.28
function
mother cell metabolism
product
methylisocitrate lyase
essential
no
ec
4.1.3.30
synonyms
mmgF, yqiQ, prpB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2513 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,507,298 2,508,203
The protein
Catalyzed reaction/ biological activity
2-methyl-isocitrate --> pyruvate + succinate [pubmed|28956599]
3-hydroxybutane-1,2,3-tricarboxylate --> pyruvate + succinate (according to UniProt)
Protein family
isocitrate lyase/PEP mutase superfamily (single member, according to UniProt)
Structure
[PDB|1MUM] (enzyme of ''E. coli''), [PDB|3KZ2] (the enzyme from ''B. anthracis'')
[AF|P54528]
Expression and Regulation
Operons
genes
[gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]-[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]-[gene|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC]-[gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]-[gene|602EAB591A550F68C14F35F6E5F0A91C3B997343|mmgE]-[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]
description
[Pubmed|8759838]
regulation
expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|15699190,8759838]
strongly expressed during oligotrophic growth [pubmed|30792386]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8759838], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,8759838], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-C374 (yqiQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2372 NBRP B. subtilis, Japan]
BKE24120 ([gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
BKK24120 ([gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGACATGAAACAAAATCCCC, downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
References
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Time of last update: 2024-12-21 14:42:57
Author of last update: Melvin.boenninger