mmgF

mmgF
168

methylisocitrate lyase

locus
BSU_24120
Molecular weight
32.94 kDa
pI
5.28
Protein length
Gene length
function
mother cell metabolism
product
methylisocitrate lyase
essential
no
ec
4.1.3.30
synonyms
mmgF, yqiQ, prpB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2513 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,507,298  2,508,203
The protein
Catalyzed reaction/ biological activity
2-methyl-isocitrate --> pyruvate + succinate [pubmed|28956599]
3-hydroxybutane-1,2,3-tricarboxylate --> pyruvate + succinate (according to UniProt)
Protein family
isocitrate lyase/PEP mutase superfamily (single member, according to UniProt)
Structure
[PDB|1MUM] (enzyme of ''E. coli''), [PDB|3KZ2] (the enzyme from ''B. anthracis'')
[AF|P54528]
Expression and Regulation
Operons
genes
[gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]-[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]-[gene|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC]-[gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]-[gene|602EAB591A550F68C14F35F6E5F0A91C3B997343|mmgE]-[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]
description
[Pubmed|8759838]
regulation
expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|15699190,8759838]
strongly expressed during oligotrophic growth [pubmed|30792386]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8759838], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,8759838], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
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[gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]→[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]

2024-12-21 13:16:32

Jstuelk

99

be5c5568e93dc96bd335d396c9ced168a6874b5d

43FB5BE81B1A707E4F1C97099736CD2DD49A154C

Biological materials
Mutant
MGNA-C374 (yqiQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2372 NBRP B. subtilis, Japan]
BKE24120 ([gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGACATGAAACAAAATCCCC,  downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
BKK24120 ([gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGACATGAAACAAAATCCCC,  downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
References
12706720,8759838,15699190,28956599

2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C

Page visits: 4576

Time of last update: 2024-12-21 14:42:57

Author of last update: Melvin.boenninger