nasA
168
[metabolite|nitrate] transporter
locus
BSU_03330
Molecular weight
45.91 kDa
pI
10.49
function
[metabolite|nitrate] uptake
product
[metabolite|nitrate] transporter
essential
no
synonyms
nasA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2223 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
362,937 364,142
The protein
Protein family
[wiki|major facilitator superfamily] (according to UniProt)
Nitrate/nitrite porter (TC 2.A.1.8) family (with [protein|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK], according to UniProt)
Structure
[PDB|4U4T] (NarK from E. coli, 24% identity) [pubmed|25959928]
[AF|P42432]
Paralogous protein(s)
[protein|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|2DE7BEC614129384779F4C761E0792385DECC563|nasA]
description
[pubmed|22383849]
regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]) [Pubmed|8799114]
expression in biofilms is organized in a ring-like pattern [pubmed|34995513]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
Biological materials
Mutant
1A972 ( ''nasA''::''phleo''), [Pubmed|7868621], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A972&Search=1A972 BGSC]
BKE03330 ([gene|2DE7BEC614129384779F4C761E0792385DECC563|nasA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATTCCCCTTTCTAG, downstream forward: _UP4_TAGGATTTTGACGGACACGC
BKK03330 ([gene|2DE7BEC614129384779F4C761E0792385DECC563|nasA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATTCCCCTTTCTAG, downstream forward: _UP4_TAGGATTTTGACGGACACGC
References
Reviews
Original Publications
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Time of last update: 2024-12-21 16:49:20
Author of last update: Jstuelk