rhiF
168
[wiki|ABC transporter] (membrane protein) for rhamnose oligosaccharides
locus
BSU_06980
Molecular weight
34.46 kDa
pI
9.47
function
uptake of rhamnose oligosaccharides
product
rhamnose oligosaccharide [wiki|ABC transporter]
essential
no
synonyms
rhiF, yesP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
762,942 763,871
The protein
Protein family
[wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
[wiki|MalFG subfamily] (according to UniProt)
[wiki|Domains]
[wiki|ABC transmembrane type-1 domain] (aa 80-294) (according to UniProt)
Structure
[PDB|4TQU] (from Sphingomonas sp., corresponds to aa 13 - 231, 28% identity) [pubmed|26235029]
[AF|O31519]
[wiki|Localization]
cell membrane [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|A96A83684E1E6E8705265D3932DA60FF47C23E3B|rhiL]-[gene|2DC415DCC2ECC3B46526108638DDB46C28832041|rhiF]-[gene|0B11FACEB5E96D166AC1EA0E32C68FCDAF8327E4|rhiG]-[gene|8EED04FCE6E2900B729178209FB281F5463B2540|rhiN]-[gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
description
[Pubmed|22383849]
regulation
induced by pectin ([protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR], [protein|F09661C8A483D0FC796204102021104A4373F895|rhgL]) [Pubmed|19651770,35881471]
repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [pubmed|35881471]
regulatory mechanism
[protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]: activation, [Pubmed|19651770], in [regulon|protein:BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: Sigma factor, [pubmed|35881471], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|F09661C8A483D0FC796204102021104A4373F895|rhgL]: activation, [pubmed|35881471], in [regulon|protein:F09661C8A483D0FC796204102021104A4373F895|rhgL regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|35881471], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Biological materials
Mutant
MGNA-A948 (yesP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/948 NBRP B. subtilis, Japan]
BKE06980 ([gene|2DC415DCC2ECC3B46526108638DDB46C28832041|rhiF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCGTCAGCCCCATTTC, downstream forward: _UP4_TACGAGTCAAAGGAGGAATG
BKK06980 ([gene|2DC415DCC2ECC3B46526108638DDB46C28832041|rhiF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCGTCAGCCCCATTTC, downstream forward: _UP4_TACGAGTCAAAGGAGGAATG
References
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Time of last update: 2024-12-21 11:58:42
Author of last update: Jstuelk