yhfK

yhfK
168

ene reductase, detoxification of α,β-unsaturated aldehydes

Locus
BSU_10260
Molecular weight
22.61 kDa
Isoelectric point
5.18
Protein length
214 aaSequenceBlast
Gene length
645 bpSequenceBlast
Function
detoxification of α,β-unsaturated aldehydes
Product
ene reductase
Essential
no
Synonyms
yhfK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0702 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,100,219  1,100,863
Phenotypes of a mutant
increased sensitivity to trans-cinnamaldehyde and several other α,β-unsaturated aldehydes, including trans-2-hexenal, citral, and 4-hydroxy-2-nonenal PubMed
The protein
Catalyzed reaction/ biological activity
trans-cinnamaldehyde NAD(P)H --> 3-phenylpropanal + NAD(P)+ by reducing the C=C double bond of the α,β-unsaturated aldehyde moiety PubMed
Protein family
NAD(P)-dependent epimerase/dehydratase family (according to UniProt)
Structure
3E8X (PDB) (from B. halodurans, 42% identity)
O07609 (AlphaFold)
Modification
phosphorylated on ser/ thr/ tyr PubMed
Expression and Regulation
Operons
Genes
yhfI-lplJ-yhfK
Description
PubMed
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yhfI→yhfK

2025-04-09 18:54:48

Jstuelk

98

b8823528516f22fffa10ba8fffe4e79b3d5468b4

CFCEBE535A67CD8FED72C985A24AEAD6F25506F0

Biological materials
Mutant
MGNA-A711 (yhfK::erm), available at the NBRP B. subtilis, Japan
BKE10260 (yhfK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAATCCCTCCTGCCT,  downstream forward: _UP4_TGACAGTACTGACACTCAGG
BKK10260 (yhfK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAATCCCTCCTGCCT,  downstream forward: _UP4_TGACAGTACTGACACTCAGG
References
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Time of last update: 2025-04-14 10:56:38

Author of last update: Jstuelk