yhfK
168
ene reductase, detoxification of α,β-unsaturated aldehydes
locus
BSU_10260
Molecular weight
22.61 kDa
pI
5.18
function
detoxification of α,β-unsaturated aldehydes
product
ene reductase
essential
no
synonyms
yhfK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0702 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,100,219 1,100,863
Phenotypes of a mutant
increased sensitivity to trans-cinnamaldehyde and several other α,β-unsaturated aldehydes, including trans-2-hexenal, citral, and 4-hydroxy-2-nonenal [pubmed|37036358]
The protein
Catalyzed reaction/ biological activity
trans-cinnamaldehyde NAD(P)H --> 3-phenylpropanal + NAD(P)+ by reducing the C=C double bond of the α,β-unsaturated aldehyde moiety [pubmed|37036358]
Protein family
[wiki|NAD(P)-dependent epimerase/dehydratase family] (according to UniProt)
Structure
[PDB|3E8X] (from B. halodurans, 42% identity)
[AF|O07609]
Modification
phosphorylated on ser/ thr/ tyr [Pubmed|16493705]
Expression and Regulation
Operons
genes
[gene|8FA225FA26DB0B89812A835EB928F2C8892EBC89|yhfI]-[gene|F68611DF326880FCCB4EE3C2B364ECCC3DEAD78D|lplJ]-[gene|2DA0641FA6EC81072DA585D65FE17B7E5A010AC9|yhfK]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A711 (yhfK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/711 NBRP B. subtilis, Japan]
BKE10260 ([gene|2DA0641FA6EC81072DA585D65FE17B7E5A010AC9|yhfK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAATCCCTCCTGCCT, downstream forward: _UP4_TGACAGTACTGACACTCAGG
BKK10260 ([gene|2DA0641FA6EC81072DA585D65FE17B7E5A010AC9|yhfK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAATCCCTCCTGCCT, downstream forward: _UP4_TGACAGTACTGACACTCAGG
References
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Time of last update: 2024-12-31 06:45:54
Author of last update: Jstuelk