ald
168
L-alanine dehydrogenase
locus
BSU_31930
Molecular weight
39.53 kDa
pI
5.12
function
alanine utilization
product
L-alanine dehydrogenase
essential
no
ec
1.4.1.1
synonyms
ald, spoVN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0686 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,278,325 3,279,461
Phenotypes of a mutant
[wiki|sporulation] defect [Pubmed|22797752]
premature spore germination due to alanine accumulation, activation of germination via [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[protein|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC] [pubmed|38564667]
The protein
Catalyzed reaction/ biological activity
H2O + L-alanine + NAD+ --> H+ + NADH + NH4+ + pyruvate (according to UniProt)
Protein family
AlaDH/PNT family (single member, according to UniProt)
[wiki|Cofactors]
NADH [Pubmed|26202482]
Structure
[PDB|2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)
[AF|Q08352]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]-[gene|F001D5A254F0E175B4B3A0B54FC4B40AA230997E|yukJ]
description
[pubmed|22383849]
genes
[gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]
description
[Pubmed|8226620]
regulation
induced by alanine ([protein|search|AdeR]) [Pubmed|22797752,8226620]
regulatory mechanism
[protein|A987E1510578E8DE822C2019488D457CB7CFBE73|adeR]: activation, [Pubmed|22797752], in [regulon|protein:A987E1510578E8DE822C2019488D457CB7CFBE73|adeR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8226620], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
Biological materials
Mutant
GP3596 (Δ[gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]::''tet''), available in [wiki|Jörg Stülke]'s lab
BKE31930 ([gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA, downstream forward: _UP4_TAATTCACAATAAGCTTGCA
BKK31930 ([gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGTCTCCTCCTGTA, downstream forward: _UP4_TAATTCACAATAAGCTTGCA
Expression vectors
pGP4001: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
FLAG-tag construct
GP4486 [gene|2D346CF412BA32EE18111DA930CFA1306F95024B|ald]-3xFLAG spc (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
Page visits: 4891
Time of last update: 2025-01-21 18:41:55
Author of last update: Robert.warneke