yfkJ
168
general stress protein, protein tyrosine phosphatase, required for protection against paraquat stress
locus
BSU_07880
Molecular weight
17.13 kDa
pI
4.56
function
survival of stress conditions
product
protein tyrosine phosphatase
essential
no
ec
3.1.3.48
synonyms
yfkJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0394 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
862,004 862,474
The protein
Catalyzed reaction/ biological activity
H2O + O-phospho-L-tyrosyl-[protein] --> L-tyrosyl-[protein] + phosphate (according to UniProt)
Protein family
low molecular weight phosphotyrosine protein phosphatase family (with [protein|DB3527FB78D0B7C144D1699D665788184F19AFE0|arsC] and [protein|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE], according to UniProt)
Structure
[PDB|4ETM]
[AF|O35016]
Paralogous protein(s)
[protein|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]
Expression and Regulation
Operons
genes
[gene|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|yfkJ]-[gene|A38D85EE1BCCA5E5E77CAF9583E7D052BB7F8188|yfkI]-[gene|0A9352AB10C7DC06BF477EBE384D6E21D920AAC8|yfkH]
description
[Pubmed|22383849]
regulation
induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [Pubmed|15805528,11532142]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11532142], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528,11532142], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-C266 (yfkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2264 NBRP B. subtilis, Japan]
BKE07880 ([gene|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|yfkJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTGCAACCTCCCTA, downstream forward: _UP4_TTGTGAGTGAAAGGAGAATT
BKK07880 ([gene|2CEFD8D9692D062CC09E3DEDA1A4015C76BC4167|yfkJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTGCAACCTCCCTA, downstream forward: _UP4_TTGTGAGTGAAAGGAGAATT
References
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Time of last update: 2024-12-21 21:01:35
Author of last update: Melvin.boenninger