radC
168
probable DNA repair protein
locus
BSU_28040
Molecular weight
26.00 kDa
pI
7.83
function
DNA repair
product
probable DNA repair protein
essential
no
synonyms
radC, ysxA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2003 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,861,840 2,862,535
The protein
Protein family
UPF0758 family (single member, according to UniProt)
[wiki|Domains]
MPN domain (aa 109-231) (according to UniProt)
Structure
[PDB|2QLC] (C-terminal domain of Chlorobium tepidum RadC, 46% identity)
[AF|Q02170]
Modification
phosphorylated on Arg-59 [Pubmed|22517742]
[wiki|Localization]
cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP3561 (Δ[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]::aphA3), available in [wiki|Jörg Stülke]'s lab
MGNA-B011 (ysxA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1010 NBRP B. subtilis, Japan]
BKE28040 ([gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCACTCCTTTTCTTTCC, downstream forward: _UP4_TAACACTTTTTTTTTCGTCG
BKK28040 ([gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCACTCCTTTTCTTTCC, downstream forward: _UP4_TAACACTTTTTTTTTCGTCG
References
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Time of last update: 2024-12-21 21:18:29
Author of last update: MBenda