coaBC

coaBC
168

[metabolite|coenzyme A] biosynthesis bifunctional protein CoaBC; [metabolite|phosphopantothenoyl-cysteine] synthetase/decarboxylase

locus
BSU_15700
Molecular weight
43.82 kDa
pI
6.01
Protein length
Gene length
function
biosynthesis of [metabolite|coenzyme A]
product
[metabolite|phosphopantothenoyl-cysteine] synthetase/decarboxylase
essential
yes
ec
4.1.1.36
synonyms
yloI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0452 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,642,851  1,644,071
Phenotypes of a mutant
essential [Pubmed|28189581], becomes dispensable when coenzyme A salvage pathway is active (e.g. in presence of panteth(e)ine) [pubmed|39194188]
The protein
Catalyzed reaction/ biological activity
H+ + N-4-[metabolite|phosphopantothenoyl-cysteine] --> CO2 + 4'-[metabolite|phosphopantetheine] [pubmed|39194188]
(R)-4'-[metabolite|phosphopantothenate] + [metabolite|CTP] + L-[metabolite|cysteine] --> [metabolite|CMP] + [metabolite|diphosphate] + H+ + N-4-[metabolite|phosphopantothenoyl-cysteine] [pubmed|39194188]
Protein family
N-terminal part: HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily (single member, according to UniProt)
C-terminal part: PPC synthetase family (single member, according to UniProt)
Structure
[PDB|6TGV] (from Mycobacterium smegmatis, 38.8% identity) [pubmed|33420031]
[PDB|5INT] (from ''Bacillus anthracis'', 60% identity in 52% C-terminal fragment)
[AF|O35033]
Expression and Regulation
Operons
genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
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[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]->[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-10-02 03:18:54

Jstuelk

129

67080d1e476b4c1544f46abe5db9b45c3ccd93f3

2C39B75EDCD294B0CAC1DECB4007285A35FDAE1D

Biological materials
Mutant
GP4670 (trpC2 Δ[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]::spc), available in [wiki|Jörg Stülke]'s lab [pubmed|39194188]
GP4681 (trpC2 Δ[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]::cat), available in [wiki|Jörg Stülke]'s lab
MGNA-B372 (yloI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1371 NBRP B. subtilis, Japan]
References
22960854,33420031,28189581,39194188

2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C

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Time of last update: 2024-10-04 07:30:42

Author of last update: Jstuelk