rfaB
168
similar to alkaline-shock protein
locus
BSU_15830
Molecular weight
13.05 kDa
pI
7.08
function
unknown
product
unknown
essential
no
synonyms
yloU
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1302 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,656,064 1,656,426
The protein
Protein family
asp23 family (with [protein|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ], according to UniProt)
Structure
[AF|O34318]
Paralogous protein(s)
[protein|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ]
Expression and Regulation
Operons
genes
[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B375 (yloU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1374 NBRP B. subtilis, Japan]
GP1465 (''yloU''-''cat ''), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
GP1467 (''yloU''-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]-''cat ''), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
BKE15830 ([gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA, downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT
BKK15830 ([gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA, downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT
Expression vectors
GP1476 (chromosomal ''yloU''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP1330 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP1324 (N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP3620: expression of Strep-[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB] by [wiki|pGP380] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3621: expression of [gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|rfaB]-Strep by [wiki|pGP382] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3629 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
pGP3638: expression in B. subtilis (based on [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP3645 (N-terminal GST-Tag, purification from ''E. coli'', in [wiki|pGEX-6P-1]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP3792 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1472 (spc, based on [wiki|pBP43]), available in [wiki|Jörg Stülke]'s lab [pubmed|28579978]
References
Reviews
Original Publications
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Time of last update: 2024-11-21 02:52:31
Author of last update: Dennis.wicke