racX
168
amino acid racemase, production of non-canonical D-amino acids
locus
BSU_34430
Molecular weight
25.14 kDa
pI
5.4
function
production of non-canonical D-amino acids
product
amino acid racemase
essential
no
synonyms
racX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1794 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,533,419 3,534,102
The protein
Catalyzed reaction/ biological activity
L-α-amino acid --> D-α-amino acid (according to UniProt)
LL-2,6-diaminoheptanedioate --> meso-2,6-diaminopimelate(according to UniProt)
L-lysine --> D-lysine (according to UniProt)
L-arginine --> D-arginine (according to UniProt)
L-ornithine --> D-ornithine (according to UniProt)
L-histidine --> D-histidine (according to UniProt)
L-alanine --> D-alanine (according to UniProt)
L-tyrosine --> D-tyrosine (according to UniProt)
L-phenylalanine --> D-phenylalanine (according to UniProt)
L-serine --> D-serine (according to UniProt)
L-glutamine --> D-glutamine (according to UniProt)
L-methionine --> D-methionine (according to UniProt)
L-asparagine --> D-asparagine (according to UniProt)
L-homoserine --> D-homoserine (according to UniProt)
Protein family
aspartate/glutamate racemases family (with [protein|36FF9F097682D8BA8D7359BFEAF71ACE8E9E90D8|racE] and [protein|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC], according to UniProt)
Structure
[PDB|1JFL] (from Pyrococcus horikoshii, 30% identity) [pubmed|12051922]
[AF|P32960]
Expression and Regulation
Operons
genes
[gene|FE672C27CF019E18A526DE8E6779788DDE54056E|pbpE]-[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]
description
[Pubmed|8491712]
regulation
induced by cell wall stress ([protein|search|SigW]) [Pubmed|8491712,12207695]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|8491712,12207695], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
Biological materials
Mutant
GP3650 (Δ[gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
BKE34430 ([gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGATCATCAAATTCATTC, downstream forward: _UP4_TAGCTTCAGACAAGGACTGT
BKK34430 ([gene|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGATCATCAAATTCATTC, downstream forward: _UP4_TAGCTTCAGACAAGGACTGT
Expression vectors
pGP3816 (expression of [protein|2ABCC493C6CA847A7BC65B1200A9B6490621462A|racX] in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-05 14:37:19
Author of last update: MBenda