ribH
168
riboflavin synthase (beta subunit)
locus
BSU_23250
Molecular weight
16.14 kDa
pI
5.2
function
riboflavin biosynthesis
product
riboflavin synthase (beta subunit)
essential
no
ec
2.5.1.9
synonyms
ribH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0054 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,427,892 2,428,356
The protein
Catalyzed reaction/ biological activity
(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil --> 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ + 2 H2O + phosphate (according to UniProt)
Protein family
DMRL synthase family (single member, according to UniProt)
Structure
[PDB|1ZIS], [PDB|1RVV] (the [protein|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE])3-([protein|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH])60 lumazine synthase/riboflavin synthase complex) [Pubmed|7473709]
[AF|P11998]
Modification
acetylyated on K29 by [protein|8146A052E4E7844F781E7169C264EC927DEB7387|ribT] [pubmed|35495702]
[wiki|Localization]
cytoplasm [pubmed|34474681]
Expression and Regulation
Operons
genes
[gene|3DFCF7C5FE15C9A3E9E7E4B0DA8E926B85DBD180|ribD]-[gene|4E18BD4B084094EE4FBC8FE2AC95B14581D20C0F|ribE]-[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]-[gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]-[gene|8146A052E4E7844F781E7169C264EC927DEB7387|ribT]
description
[Pubmed|8159171]
regulation
expressed in the absence of FMN ([wiki|FMN-box]) [Pubmed|15808508]
binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR] to the [wiki|FMN-box] riboswitch can enforce expression even in the presence of FMN [pubmed|26494285]
the [wiki|FMN-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
FMN-box: RNA switch, via [wiki|FMN-box] in the presence of FMN or FMNH2, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR], in [regulon|other_regulator:FMN-box|FMN-box]
[protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]: antitermination, [pubmed|26494285], in [regulon|protein:3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8159171], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE23250 ([gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATTCCCATCCTTTT, downstream forward: _UP4_TAATTTGCTGAAAACAGTTT
BKK23250 ([gene|2A5DCBFAB51996F0DDEC964D5D178274AF86CE69|ribH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATTCCCATCCTTTT, downstream forward: _UP4_TAATTTGCTGAAAACAGTTT
References
Reviews
Original Publications
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Time of last update: 2024-11-03 01:47:52
Author of last update: Jstuelk