ilvE
168
branched-chain amino acid aminotransferase
locus
BSU_02390
Molecular weight
39.52 kDa
pI
4.95
function
biosynthesis of branched-chain amino acids
product
branched-chain amino acid aminotransferase
essential
no
ec
2.6.1.42
synonyms
ybgE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0115 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
259,016 260,086
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + L-leucine --> 4-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-isoleucine --> (S)-3-methyl-2-oxopentanoate + L-glutamate (according to UniProt)
2-oxoglutarate + L-valine --> 3-methyl-2-oxobutanoate + L-glutamate (according to UniProt)
Protein family
[wiki|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|3HT5] (from ''Mycobacterium tuberculosis'', 48% identity) [Pubmed|19923721]
[AF|O31461]
Paralogous protein(s)
[protein|8163996FCB9D02F771E7A04A91D5720027260F12|ilvK]
Expression and Regulation
Operons
genes
[gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE]
description
[Pubmed|15060025]
regulation
repressed by casamino acids [Pubmed|12107147]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455,21699902,18083814], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
Biological materials
Mutant
MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]
a ''ybgE'' mutant and a ''[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd] [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE] [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ilvK]'' triple mutant are available in [wiki|Linc Sonenshein]'s lab
BKE02390 ([gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
BKK02390 ([gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ilvE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
lacZ fusion
pGP248 (in [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2025-04-03 20:14:40
Author of last update: Robert.warneke