spo0B
168
[category|SW.4.2|Sporulation] initiation phosphotransferase of the [wiki|phosphorelay]
locus
BSU_27930
Molecular weight
22.40 kDa
pI
4.96
function
initiation of [wiki|sporulation]
product
[wiki|sporulation ]initiation phosphotransferase
essential
no
synonyms
spo0B, spo0D
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5802 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,853,981 2,854,559
Phenotypes of a mutant
Arrest of [wiki|sporulation ]at stage 0 (initiation) [pubmed|4633157]
The protein
Structure
[PDB|1F51] (complex with [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F])
[PDB|1IXM]
[AF|P06535]
Expression and Regulation
Operons
genes
[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2537815], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
expression is repressed by binding of [wiki|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
Biological materials
Mutant
[http://www.ncbi.nlm.nih.gov/nuccore/254763670 spo0B12], point mutation 85C>T (=R29W), available in [http://bgsc.org/ BGSC] 1S54
[http://www.ncbi.nlm.nih.gov/nuccore/254763672 spo0B136], amber mutation 103A>T (=K35X), available in [http://bgsc.org/ BGSC] 1S16
[http://www.ncbi.nlm.nih.gov/nuccore/254763674 spo0B580ts], point mutation 193G>A (=E65K), available in [http://bgsc.org/ BGSC] 1S90
[http://www.ncbi.nlm.nih.gov/nuccore/254763676 spo0B581ts], point mutation 461G>A (=G154D), available in [http://bgsc.org/ BGSC] 1S91
BKE27930 ([gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCGCACTCCCAATCA, downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT
BKK27930 ([gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCGCACTCCCAATCA, downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT
References
Reviews
Original Publications
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Time of last update: 2024-12-21 10:18:41
Author of last update: Jstuelk