alkA

alkA
168

DNA-3-methyladenine glycosylase

locus
BSU_01800
Molecular weight
35.48 kDa
pI
8.15
Protein length
Gene length
function
adaptive response to alkylative DNA damage
product
DNA-3-methyladenine glycosylase
essential
no
ec
3.2.2.21
synonyms
alkA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0122 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
202,547  203,458
The protein
Catalyzed reaction/ biological activity
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)
Protein family
alkylbase DNA glycosidase AlkA family (with [protein|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP], according to UniProt)
Structure
[PDB|2JHJ] (from Archaeoglobus fulgidus, 29% identity) [pubmed|17396151]
[AF|P37878]
Expression and Regulation
Operons
genes
[gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]
description
[Pubmed|8376346]
regulatory mechanism
[protein|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]: activation, in [regulon|protein:0F8A564256A598DB3A36668FA0C984542FE1323F|adaA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8376346], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]

2024-10-03 14:10:43

ghost

70

676a0907f9180d6e66d70f7c75e6e39c4b25e7f0

53C1A72D1C318FDC32AFE83167CE6185E9FCC428

Biological materials
Mutant
BKE01800 ([gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT,  downstream forward: _UP4_TGAATCATTTCAACTTTAAC
BKK01800 ([gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT,  downstream forward: _UP4_TGAATCATTTCAACTTTAAC
References
Reviews
22933559,24810496,35210609
Original Publications
8376346,17396151

297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509

Page visits: 2770

Time of last update: 2024-10-02 13:38:45

Author of last update: Jstuelk