fusA
168
elongation factor G, catalyzes translocation steps of the ribosome after peptide bond formation, facilitates movement of tRNA and mRNA by one codon
locus
BSU_01120
Molecular weight
76.36 kDa
pI
4.61
function
translation
product
elongation factor G
essential
yes
synonyms
fusA, fus
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0480 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
130,684 132,762
Phenotypes of a mutant
essential [Pubmed|12682299]
specific point mutations affecting [protein|297844645CCCF40CB40E84339997F6610883DF4E|fusA] facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
The protein
Catalyzed reaction/ biological activity
hydrolyses GTP
catalyzes translocation steps of the ribosome after peptide bond formation, facilitates movement of tRNA and mRNA by one codon [pubmed|20025795]
Protein family
[wiki|TRAFAC class translation factor GTPase superfamily] (according to UniProt)
[wiki|Classic translation factor GTPase family] (according to UniProt)
[wiki|Domains]
[wiki|tr-type G domain] (aa 8-282) (according to UniProt)
Structure
[PDB|1ELO] (from ''Thermus thermophilus'') [Pubmed|8070397]
[PDB|4BTC], [PDB|4BTD], the ''Thermus thermophilus'' EF-G[wiki|ribosome] complex in a pretranslocation state [Pubmed|23912278]
[AF|P80868]
Modification
phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [Pubmed|17218307], [Pubmed|17726680]
phosphorylated on Tyr-339 [Pubmed|26381121]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
description
[pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP840, available in [wiki|Jrg Stlke]'s lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP848, available in [wiki|Jrg Stlke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-20 04:31:20
Author of last update: Jstuelk