yabJ

yabJ
168

2-iminobutanoate/2-iminopropanoate deaminase, detoxification of reactive intermediates generated in isoleucine biosynthesis

Locus
BSU_00480
Molecular weight
13.51 kDa
Isoelectric point
5.13
Protein length
125 aaSequenceBlast
Gene length
378 bpSequenceBlast
Function
deamination of reactive intermediates generated in isoleucine biosynthesis
Product
2-iminobutanoate/2-iminopropanoate deaminase
Essential
no
Synonyms
yabJ, ridA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0251 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
55,295  55,672
The protein
Catalyzed reaction/ biological activity
release of ammonia from reactive enamine/imine intermediates of the pyridoxal 5'-phosphate-dependent threonine dehydratase (IlvA) PubMed
2-iminobutanoate + H2O = 2-oxobutanoate + NH3 PubMed
2-iminopropanoate + H2O = pyruvate + NH3 PubMed
Protein family
RutC family (single member, according to UniProt)
Structure
1QD9 (PDB) NCBI PubMed
5Y6U (PDB)
P37552 (AlphaFold)
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
ispE-purR-yabJ
Description
PubMed
Regulation
negative autoregulation PubMed
Regulatory mechanism
PurR: repression, PubMed, in purR regulon
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ispE→yabJ

2025-03-31 01:13:41

Jstuelk

115

620be59868c14a2ea15f75e6fdf1022628ca65bb

B42ADE39EB92F53783BD0BDEF129E37859FEBF47

Additional information
expression is reduced in motile cells as compared to non-motile cells PubMed
Biological materials
Mutant
MGNA-B911 (yabJ::erm), available at the NBRP B. subtilis, Japan
BKE00480 (yabJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT,  downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
BKK00480 (yabJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT,  downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
References
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Time of last update: 2025-04-10 10:22:41

Author of last update: Jstuelk