yabJ
168
2-iminobutanoate/2-iminopropanoate deaminase, detoxification of reactive intermediates generated in [metabolite|isoleucine] biosynthesis
locus
BSU_00480
Molecular weight
13.51 kDa
pI
5.13
function
deamination of reactive intermediates generated in [metabolite|isoleucine] biosynthesis
product
2-iminobutanoate/2-iminopropanoate deaminase
essential
no
synonyms
yabJ, ridA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0251 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
55,295 55,672
The protein
Catalyzed reaction/ biological activity
release of ammonia from reactive enamine/imine intermediates of the pyridoxal 5'-phosphate-dependent threonine dehydratase ([protein|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|ilvA]) [pubmed|22094463]
2-iminobutanoate + H2O = 2-oxobutanoate + NH3 [pubmed|22094463]
2-iminopropanoate + H2O = pyruvate + NH3 [pubmed|22094463]
Protein family
RutC family (single member, according to UniProt)
Structure
[PDB|1QD9] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=11484 NCBI] [Pubmed|10557275]
[PDB|5Y6U]
[AF|P37552]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
description
[Pubmed|22383849]
regulation
negative autoregulation [Pubmed|7638212]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [Pubmed|7638212], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
additional information
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
Biological materials
Mutant
MGNA-B911 (yabJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1910 NBRP B. subtilis, Japan]
BKE00480 ([gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT, downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
BKK00480 ([gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT, downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
References
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Time of last update: 2024-12-20 14:49:30
Author of last update: Jstuelk