sunT
168
[protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|sublancin] 168 lantibiotic [wiki|ABC transporter]
locus
BSU_21470
Molecular weight
81.38 kDa
pI
8.39
function
[protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|sublancin] export and processing
product
[protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|sublancin] 168 lantibiotic [wiki|ABC transporter]
essential
no
synonyms
sunT, yolH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2274 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,267,346 2,269,463
The protein
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
Peptidase C39 domain (aa 12-138) (according to UniProt)
[wiki|ABC transmembrane type-1 domain] (aa 168-450) (according to UniProt)
[wiki|ABC transporter domain] (aa 483-705) (according to UniProt)
Structure
[PDB|4RY2] (from Clostridium thermocellum, 33% identity) [pubmed|26201595]
[AF|P68579]
[wiki|Localization]
membrane [Pubmed|10092453]
Expression and Regulation
Operons
genes
[gene|1A6D90298D039FFFD977B2534952BA5E32B3530F|sunA]-[gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]-[gene|DB5F719C0A2F610301E174F6890DA9E826C76932|bdbA]-[gene|6B2DC9873B7B693D9D0FC0D773740140D0640C9E|sunS]-[gene|12CE5AE0BD02C989E803480A1CE5A47D6192D751|bdbB]
description
[Pubmed|22383849]
regulation
repressed by [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]-[wiki|DnaA] [Pubmed|27902860,15743949]
expression starts in the stationary phase [pubmed|30808982]
expression is heterogeneous in the population, this is mediated by [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB], [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok], and [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] [pubmed|30808982]
regulatory mechanism
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
[protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: activation, [Pubmed|20817675,19465659], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|27902860,15743949], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
[protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: activation, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675,17720793], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
the amount of the mRNA is substantially decreased upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
GP3432 (Δ[gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]::spec) , available in [wiki|Jörg Stülke]'s lab
BKE21470 (''sunT''::''erm trpC2'') available in the Bacillus Genetic Stock Center and in [wiki|Jörg Stülke]'s lab [pubmed|28189581]
BKE21470 ([gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTCAATCCCCGCTTTA, downstream forward: _UP4_TCGGAAAATAAGGAGTATTC
BKK21470 ([gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTCAATCCCCGCTTTA, downstream forward: _UP4_TCGGAAAATAAGGAGTATTC
References
Reviews
Original Publications
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Time of last update: 2024-12-21 01:30:02
Author of last update: Jstuelk