epeE
168
radical SAM epimerase, modification of [protein|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]
locus
BSU_40170
Molecular weight
37.06 kDa
pI
5.86
function
control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]-[protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS] activity
product
radical SAM epimerase
essential
no
synonyms
yydG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0535 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,126,481 4,127,440
The protein
Catalyzed reaction/ biological activity
epimerization of Val-36 and Ile-44 of [protein|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX] to the D-form [pubmed|28644475]
Protein family
radical SAM enzymes [pubmed|28644475]
[wiki|Cofactors]
two Fe-S clusters [pubmed|28644475]
SAM [pubmed|28644475]
Structure
[AF|Q45595]
Expression and Regulation
Operons
genes
[gene|D66B9BFF79ADD8B0F9BFA3892FCF4597264CE9EE|epeX]-[gene|25FC87037222084ABE318813959101016FEB49DF|epeE]-[gene|E280A5994F4D9D93DA4B698E79C86360DBEE5396|epeP]-[gene|1BF1A48CF645249CA9ECBB19C04B059B11D821EF|epeA]-[gene|A4213DE6C9BE29405B0A4C0A969A78EA4C5EB71F|epeB]
description
[Pubmed|17921301]
regulation
repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|17921301]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|34120110,17921301], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|15743949], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17921301], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B810 (yydG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1809 NBRP B. subtilis, Japan]
BKE40170 ([gene|25FC87037222084ABE318813959101016FEB49DF|epeE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATATCCCCCAACAA, downstream forward: _UP4_TATCCTTATATGGAAAAATA
BKK40170 ([gene|25FC87037222084ABE318813959101016FEB49DF|epeE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATATCCCCCAACAA, downstream forward: _UP4_TATCCTTATATGGAAAAATA
References
Reviews
Original Publications
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Time of last update: 2024-10-15 04:25:23
Author of last update: Jstuelk