ymfI

ymfI
168

[protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]-5 aminopentanone reductase

locus
BSU_16870
Molecular weight
25.43 kDa
pI
5.22
Protein length
Gene length
function
modification of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
product
[protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]-5 aminopentanone reductase
essential
no
synonyms
ymfI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1028 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,759,655  1,760,383
Phenotypes of a mutant
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827,28787546], this can be suppressed by mutations affecting earlier steps in [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification ([gene|8BB8EECA8ECFA1CC6D46E81250905DBC6D2C503E|gsaB], [gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO], [gene|62718BBECEB18244B5CC877509679F5F9A0C50CD|yfkA], [gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB], [gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]) [pubmed|29615499]
The protein
Catalyzed reaction/ biological activity
reduction of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]-5 aminopentanone to [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]-5 aminopentanol [pubmed|28787546]
Protein family
[wiki|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)
[wiki|Cofactors]
NADPH [pubmed|28787546]
Structure
[PDB|2PNF] (from Aquifex Aeolicus , 36% identity)
[AF|O31767]
Paralogous protein(s)
[protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|yhxC], [protein|B6FF689E65906186F3576B378650D713DB84EDDA|ycdF], [protein|EAEEA4DD9641919830A81185333A2610B964D37C|yhdF]
[protein|439B468A13137000FB42E9389391CB4986FFED84|fabG], (34%)
[protein|D9D1FCBFC62F93CAA34DF61A784406F4E9EE6768|yjdA], (30,8%)
Biological materials
Mutant
MGNA-B379 (ymfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1378 NBRP B. subtilis, Japan]
BKE16870 ([gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTCTTTCCTTCTTTCC,  downstream forward: _UP4_TGATCCGAAAATATTCGGTG
BKK16870 ([gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTCTTTCCTTCTTTCC,  downstream forward: _UP4_TGATCCGAAAATATTCGGTG
References
Research papers
28787546,29615499,35638827

248F0805272FED9B38ECBB31E2872BC9EC163CE0

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Time of last update: 2024-12-05 10:44:48

Author of last update: Jstuelk