yqeK
168
diphosphatase, symmetrically cleaving Ap4A hydrolase, degradation of toxic side-products of the nicotinamide-nucleotide adenylyltransferase [protein|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]
locus
BSU_25630
Molecular weight
21.14 kDa
pI
5.97
function
degradation of Ap4A, degradation of toxic side-products of [protein|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD] activity
product
diphosphatase, Ap4A hydrolase
essential
no
synonyms
yqeK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1713 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,643,215 2,643,775
Phenotypes of a mutant
increased levels of Ap4A [pubmed|32152217]
The protein
Catalyzed reaction/ biological activity
symmetric cleavage of Ap4A [pubmed|32152217]
[wiki|Domains]
[wiki|HD domain] (aa 18-132) (according to UniProt)
Structure
[PDB|3CCG] (the protein from ''Clostridium acetobutylicum'', 46% identity, 76% similarity)
[AF|P54456]
Additional information
in some organisms [protein|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD] and [protein|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK] form one fused protein [pubmed|35862786]
Expression and Regulation
Operons
genes
[gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
description
[Pubmed|22383849]
expression
expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
Biological materials
Mutant
BKE25630 ([gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG, downstream forward: _UP4_AGCTGAACAGGAGGAATTTG
BKK25630 ([gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG, downstream forward: _UP4_AGCTGAACAGGAGGAATTTG
References
Reviews
Original Publications
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Time of last update: 2024-12-26 19:30:35
Author of last update: Jstuelk