dynA
168
dynamin-like protein, mediates membrane fusion
locus
BSU_22030
Molecular weight
137.16 kDa
pI
5.72
function
fusion of membranes
product
dynamin-like protein
essential
no
synonyms
dynA, ypbR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1159 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,312,529 2,316,110
Phenotypes of a mutant
sensitive to membrane stress-inducing antibiotics [Pubmed|26530236]
increased sensitivity to phage infection by ɸ29 and SPβ [pubmed|26530236]
The protein
Catalyzed reaction/ biological activity
mediates nucleotide independent membrane fusion ''in vitro'' [Pubmed|21205012]
causes delay of host cell lysis upon phage infection [pubmed|35164550]
[wiki|Domains]
two separate dynamin-like subunits and GTPase domains [Pubmed|21205012]
[wiki|Cofactors]
Mg(2 ) [Pubmed|21205012]
Structure
[AF|P54159]
[wiki|Localization]
associated to the membrane [Pubmed|21205012]
membrane, forms foci at the site of septation [Pubmed|23060960]
forms large, almost immobile clusters on the cell membrane upon phage infection to support membrane integrity [pubmed|35164550]
colocalizes with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|ftsZ] [Pubmed|23249255]
highly dynamic membrane-associated protein; upon membrane damage, DynA localizes into large and static assemblies [Pubmed|26530236]
Expression and Regulation
Operons
genes
[gene|22F930B371A833BAEA84B32FADBB2BBA0B91A53B|dynA]-[gene|9899D0CC5FF530A48532A653075494686796564D|ypbS]-[gene|BD8D1EBA85928C4ECA4FF50556FC7C59F9BC0E9D|ypzF]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8396117], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A877 (ypbR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/877 NBRP B. subtilis, Japan]
BKE22030 ([gene|22F930B371A833BAEA84B32FADBB2BBA0B91A53B|dynA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTCTGAACCCCTTTG, downstream forward: _UP4_TAAAATTCAGTTGGCTTGTA
BKK22030 ([gene|22F930B371A833BAEA84B32FADBB2BBA0B91A53B|dynA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTCTGAACCCCTTTG, downstream forward: _UP4_TAAAATTCAGTTGGCTTGTA
References
Reviews
Original Publications
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Time of last update: 2024-10-06 08:23:30
Author of last update: Jstuelk