accA
168
acetyl-CoA carboxylase (alpha subunit)
locus
BSU_29200
Molecular weight
36.18 kDa
pI
6.09
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (alpha subunit))
essential
yes
ec
6.4.1.2
synonyms
accA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0825 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,987,731 2,988,708
Phenotypes of a mutant
essential [Pubmed|12682299]
specific point mutations affecting [protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA] facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
additional information
[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA], [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], and [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB], [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC], [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD] or downregulation of the [wiki|FapR regulon] due to specific mutations in [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [pubmed|37017514]
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + N6-carboxybiotinyl-L-lysyl-[protein] --> malonyl-CoA + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
accA family (single member, according to UniProt)
[wiki|Domains]
CoA carboxyltransferase C-terminal domain (aa 38-292) (according to UniProt)
Structure
[PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], 64% identity) [Pubmed|16460018]
[AF|O34847]
[wiki|Localization]
cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
description
[Pubmed|22383849]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
Biological materials
Expression vectors
pGP1021: purification from ''E. coli'' with an N-terminal His-tag, with TEV cleavage site, (in [wiki|pETM-11]), available in [wiki|Jörg Stülke]'s lab
pGP1024: purification from ''E. coli'' with an N-terminal Strep-tag, with TEV cleavage site, (in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP1723: purification from ''B. subtilis'' with an C-terminal Strep-tag, for [wiki|SPINE], (in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
pGP3696: expression of [gene|search|accDA] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1487 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1490 (spc, based on [wiki|pBP43]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
Page visits: 6047
Time of last update: 2024-11-20 20:20:11
Author of last update: Dennis.wicke