ccpN
168
transcriptional repressor (CcpN family) of gluconeogenetic genes and of [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1], repression in the presence of glucose, involved in the control of cell elongation
locus
BSU_25250
Molecular weight
23.72 kDa
pI
7.22
function
repressor of genes involved in gluconeogenesis ([gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB], [gene|74D4616D1A550D87182FAAC2A4AF6E04F81E1572|pckA]) and of [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1]
product
transcriptional regulator (CcpN family)
essential
no
synonyms
ccpN, yqzB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2905 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,604,959 2,605,597
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway [Pubmed|18586936]
The protein
Catalyzed reaction/ biological activity
transcription repression of the [gene|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB], [gene|74D4616D1A550D87182FAAC2A4AF6E04F81E1572|pckA], and [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] genes in the presence of glucose [Pubmed|2548995]
Protein family
[wiki|CcpN family]
[wiki|Domains]
[wiki|HTH deoR-type domain] (aa 6-70) (according to UniProt)
2 [wiki|CBS domain]s (aa 83-139, aa 148-211) (according to UniProt)
Structure
[PDB|3FV6] (the [wiki|CBS domain]s, aa 63 ... 212)
[AF|O34994]
Effectors of protein activity
[protein|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL] (negative effector of CcpN activity) [Pubmed|15720552]
ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN [Pubmed|18511073]
Expression and Regulation
Operons
genes
[gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]-[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]
description
[Pubmed|15720552]
regulation
constitutive [Pubmed|15720552]
additional information
the intracellular concentration of CcpN is about 4 myM (according to [PubMed|20408793]).
Biological materials
Mutant
GP3407 (Δ[gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]::kan) , available in [wiki|Jörg Stülke]'s lab
GP1678 (Δ([gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]-[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]::aphA3 trpC2), available in [wiki|Jörg Stülke]'s lab
MGNA-C493 (yqzB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2491 NBRP B. subtilis, Japan]
DB104 ''ccpN''::cat, available in [wiki|Sabine Brantl]'s lab
GP1128 ''ccpN''::cat, available in [wiki|Jörg Stülke]'s lab
BKE25250 ([gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCACCTTTTCACTTCATA, downstream forward: _UP4_TAAGATTGCAAACTAACGGG
BKK25250 ([gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCACCTTTTCACTTCATA, downstream forward: _UP4_TAAGATTGCAAACTAACGGG
Expression vectors
pGP2923 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Sabine Brantl]'s lab
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
[wiki|Sabine Brantl], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]
[wiki|Uwe Sauer], ETH Zrich, Switzerland [http://www.imsb.ethz.ch/researchgroup/sauer homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-31 06:57:28
Author of last update: Jstuelk