yngE
168
methylcrotonoyl-CoA carboxylase, subunit
locus
BSU_18210
Molecular weight
66.13 kDa
pI
8.36
function
mother cell metabolism, leucine utilization
product
methylcrotonoyl-CoA carboxylase, subunit
essential
no
ec
6.4.1.4
synonyms
yngE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4799 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,949,682 1,951,217
The protein
Catalyzed reaction/ biological activity
3-methylbut-2-enoyl-CoA + Biotin-CO2 -- 3-methyl-glutaconyl-CoA + biotin [Pubmed|19935659]
Protein family
AccD/PCCB family (with [protein|E4997526CC534E38413030E90F7A135ECF65DF40|accD] and [protein|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|yqjD], according to UniProt)
[wiki|Domains]
CoA carboxyltransferase N-terminal domain (aa 2-254) (according to UniProt)
CoA carboxyltransferase C-terminal domain (aa 260-506) (according to UniProt)
Structure
[PDB|1VRG] (from Thermotoga maritima, 40% identity)
[AF|O31825]
Paralogous protein(s)
[protein|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|yqjD]
Expression and Regulation
Operons
genes
[gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
description
[Pubmed|12662922]
regulation
expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-B081 (yngE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1080 NBRP B. subtilis, Japan]
BKE18210 ([gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAATCCATCAGCATTGCCT, downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG
BKK18210 ([gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAATCCATCAGCATTGCCT, downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG
References
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Time of last update: 2024-12-22 10:19:10
Author of last update: Melvin.boenninger